Research Advances and Detection Methodologies for Microbe-Derived Acetylcholinesterase Inhibitors: A Systemic Review

• Published in: Molecules (Basel, Switzerland) Vol. 22; no. 1; p. 176
• Main Authors: Su, Jingqian, Liu, Huiying, Guo, Kai, Chen, Long, Yang, Minhe, Chen, Qi
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 23.01.2017; MDPI
• Subjects: Acetylcholinesterase - metabolism; acetylcholinesterase inhibitors; Alkaloids - pharmacology; Alzheimer Disease - drug therapy; Alzheimer's disease; Bacteria - metabolism; Cholinesterase Inhibitors - analysis; Cholinesterase Inhibitors - isolation & purification; Cholinesterase Inhibitors - therapeutic use; Chromatography; Chromatography, Liquid - methods; Chromatography, Thin Layer - methods; Enzymes; Fungi - metabolism; in vitro assays; Laboratories; Mass spectrometry; Mass Spectrometry - methods; Myasthenia gravis; Nervous system; Reagents; Review; Scientific imaging; Solvents; Alzheimer’s disease; acetylcholinesterase inhibitors; in vitro assays
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules22010176

Acetylcholinesterase inhibitors (AChEIs) are an attractive research subject owing to their potential applications in the treatment of neurodegenerative diseases. Fungi and bacteria are major producers of AChEIs. Their active ingredients of fermentation products include alkaloids, terpenoids, phenylpropanoids, and steroids. A variety of in vitro acetylcholinesterase inhibitor assays have been developed and used to measure the activity of acetylcholinesterases, including modified Ellman’s method, thin layer chromatography bioautography, and the combined liquid chromatography-mass spectrometry/modified Ellman’s method. In this review, we provide an overview of the different detection methodologies, the microbe-derived AChEIs, and their producing strains.