Highly selectively monitoring heavy and transition metal ions by a fluorescent sensor based on dipeptide

• Published in: Talanta (Oxford) Vol. 85; no. 3; pp. 1566 - 1574
• Main Authors: Neupane, Lok Nath, Thirupathi, Ponnaboina, Jang, Sujung, Jang, Min Jung, Kim, Jung Hwa, Lee, Keun-Hyeung
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 15.09.2011; Elsevier
• Subjects: Ag(I); Analysis methods; Analytical chemistry; Applied sciences; Biosensing Techniques - methods; Chemistry; Contaminants; Copper - analysis; Copper - chemistry; Dipeptides - chemical synthesis; Dipeptides - chemistry; Drinking water; Exact sciences and technology; Fluorescence; Fluorescent; General, instrumentation; Global environmental pollution; HeLa Cells; Hg(II); Humans; Intracellular Space - chemistry; Ions - chemistry; Mercury - analysis; Mercury - chemistry; Metal ions; Metals, Heavy - chemistry; Monitoring; Natural water pollution; Peptide; Pollution; Ratiometric; Reproducibility of Results; Sensor; Sensors; Silver - analysis; Silver - chemistry; Solid phase synthesis; Solid-Phase Synthesis Techniques; Spectrometry, Fluorescence - methods; Transition Elements - chemistry; Transition metals; Utilities; Water Pollutants, Chemical - analysis; Water treatment and pollution; Fluorescent; Ag(I); Hg(II); Peptide; Ratiometric; Sensor; Copper II; Drinking water; Peptides; Metal ion; Mercury I; Detection limit; Copper I; Monitoring; Mercury II; Transition metal; Chemical sensor; Heavy metal; Silver II; Dipeptides; Affinity; Environment; Water pollution; Intracellular; Solid phase; Aqueous solution; Detection; Silver I
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2011.06.052

Fluorescent sensor (DMH) based on dipeptide was efficiently synthesized in solid phase synthesis. The dipeptide sensor shows sensitive response to Ag(I), Hg(II), and Cu(II) among 14 metal ions in 100% aqueous solution. The fluorescent sensor differentiates three heavy metal ions by response type; turn on response to Ag(I), ratiometric response to Hg(II), and turn off detection of Cu(II). The detection limits of the sensor for Ag(I) and Cu(II) were much lower than the EPA's drinking water maximum contaminant levels (MCL). Specially, DMH penetrated live cells and detected intracellular Ag+ by turn on response. We described the fluorescent change, binding affinity, detection limit for the metal ions. The study of a heavy metal-responsive sensor based on dipeptide demonstrates its potential utility in the environment field.