Degradation of Proteins and Starch by Combined Immobilization of Protease, α-Amylase and β-Galactosidase on a Single Electrospun Nanofibrous Membrane

• Published in: Molecules (Basel, Switzerland) Vol. 24; no. 3; p. 508
• Main Authors: Cloete, William J., Hayward, Stefan, Swart, Pieter, Klumperman, Bert
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 31.01.2019; MDPI
• Subjects: Biofilms; enzyme; Enzymes; Food; immobilization; Membrane reactors; poly(styrene-co-maleic anhydride); Polymers; protease; Proteins; Reagents; Value added; United States > US; Ireland; enzyme; poly(styrene-co-maleic anhydride); protease; immobilization

Two commercially available enzymes, Dextrozyme (α-amylase) and Esperase (protease), were covalently immobilized on non-woven electrospun poly(styrene-co-maleic anhydride) nanofiber mats with partial retention of their catalytic activity. Immobilization was achieved for the enzymes on their own as we...