Degradation of Proteins and Starch by Combined Immobilization of Protease, α-Amylase and β-Galactosidase on a Single Electrospun Nanofibrous Membrane

Two commercially available enzymes, Dextrozyme (α-amylase) and Esperase (protease), were covalently immobilized on non-woven electrospun poly(styrene-co-maleic anhydride) nanofiber mats with partial retention of their catalytic activity. Immobilization was achieved for the enzymes on their own as we...

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Published inMolecules (Basel, Switzerland) Vol. 24; no. 3; p. 508
Main Authors Cloete, William J., Hayward, Stefan, Swart, Pieter, Klumperman, Bert
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 31.01.2019
MDPI
Subjects
Biofilms
enzyme
Enzymes
Food
immobilization
Membrane reactors
poly(styrene-co-maleic anhydride)
Polymers
protease
Proteins
Reagents
Value added
United States > US
Ireland
enzyme
poly(styrene-co-maleic anhydride)
protease
immobilization
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Summary:Two commercially available enzymes, Dextrozyme (α-amylase) and Esperase (protease), were covalently immobilized on non-woven electrospun poly(styrene-co-maleic anhydride) nanofiber mats with partial retention of their catalytic activity. Immobilization was achieved for the enzymes on their own as well as in different combinations with an additional enzyme, β-galactosidase, on the same non-woven nanofiber mat. This experiment yielded a universal method for immobilizing different combinations of enzymes with nanofibrous mats containing maleic anhydride (MAnh) residues in the polymer backbone.
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ISSN:1420-3049
1420-3049
DOI:10.3390/molecules24030508