Leakage of heart fatty acid binding protein with ischemia and reperfusion in the rat

• Published in: Journal of molecular and cellular cardiology Vol. 21; no. 6; pp. 577 - 583
• Main Authors: Knowlton, Anne A., Apstein, Carl S., Saouf, Rola, Brecher, Peter
• Format: Journal Article
• Language: English
• Published: Kent Elsevier Ltd 01.06.1989; Elsevier
• Subjects: Animals; Biological and medical sciences; Carrier Proteins - blood; Carrier Proteins - metabolism; Coronary Disease - metabolism; Enzyme-Linked Immunosorbent Assay; Fatty acid binding protein; Fatty Acid-Binding Protein 7; Fatty Acid-Binding Proteins; fatty acids; Fundamental and applied biological sciences. Psychology; Heart; ischemia; Male; Myocardial ischemia; Myocardial metabolism; Myocardial Reperfusion Injury - metabolism; Myocardium - metabolism; Neoplasm Proteins; Nerve Tissue Proteins; Rats; Rats, Inbred Strains; Reperfusion injury; Vertebrates: cardiovascular system; Myocardial ischemia; Fatty acid binding protein; Myocardial metabolism; Reperfusion injury; Binding protein; Regulation(control); Ischemia; Myocardium; Cardiovascular disease; Metabolism; Fatty acids; ELISA assay
• ISSN: 0022-2828; 1095-8584
• DOI: 10.1016/0022-2828(89)90823-7

Fatty acids and their metabolites have been implicated as a cause of myocardial damage during ischemia. Fatty acid binding protein (FABP), an abundant low molecular weight protein present in the cytosol of myocytes, has been postulated to be a key fatty acid carrier protein in the myocardium. Postulating that loss of FABP during ischemia could cause an increase in unbound intracellular fatty acids contributing to myocardial damage, we measured plasma FABP levels during 60 min of myocardial ischemia followed by 60 min of reperfusion in the rat. Peak levels were seen 15 min after reperfusion. Plasma levels were higher with larger areas of myocardial ischemia (1720 ± 528 ng/ml vs. 216 ± 76 ng/ml with smaller areas, P < 0.02). Tissue levels after 60 min of ischemia and 60 min of reperfusion were decreased by over 50%, (1.0 ± 0.3 mg FABP/g wet wt compared with 2.9 ± 0.4 mg FABP/g wet wt in normal myocardium, P < 0.005). The data is consistent with the proposal that loss of FABP contributes to the myocardial damage associated with ischemia and reperfusion. Additional studies are needed to determine the exact role of FABP in the regulation of fatty acid metabolism in the heart.