Multiplex PCR-Ligation Detection Reaction Assay for Simultaneous Detection of Drug Resistance and Toxin Genes from Staphylococcus aureus, Enterococcus faecalis, and Enterococcus faecium

• Published in: Journal of Clinical Microbiology Vol. 48; no. 1; pp. 277 - 280
• Main Authors: Granger, K, Rundell, M.S, Pingle, M.R, Shatsky, R, Larone, D.H, Golightly, L.M, Barany, F, Spitzer, E.D
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.01.2010; American Society for Microbiology (ASM)
• Subjects: Bacterial Toxins - genetics; Bacteriological Techniques - methods; Bacteriology; Biological and medical sciences; Blood - microbiology; DNA, Bacterial - genetics; Drug Resistance, Bacterial; Enterococcus faecalis; Enterococcus faecalis - genetics; Enterococcus faecalis - isolation & purification; Enterococcus faecium; Enterococcus faecium - genetics; Enterococcus faecium - isolation & purification; Fundamental and applied biological sciences. Psychology; Gram-Positive Bacterial Infections - microbiology; Humans; Microbiology; Miscellaneous; Polymerase Chain Reaction - methods; Sensitivity and Specificity; Staphylococcus aureus; Staphylococcus aureus - genetics; Staphylococcus aureus - isolation & purification; Enterococcus faecalis; Lactic acid bacteria; Multiplex polymerase chain reaction; Resistance; Streptococcaceae; Enterococcus faecium; Toxin; Gene; Bacteria; Micrococcales; Micrococcaceae; Detection; Staphylococcus aureus
• ISSN: 0095-1137; 1098-660X
• DOI: 10.1128/JCM.01411-09

A multiplex PCR-ligation detection reaction (PCR-LDR) assay was developed for rapid detection of methicillin, tetracycline, and vancomycin resistance, as well as toxic shock toxin and Panton-Valentine leukocidin. The assay was tested on 470 positive blood culture bottles containing Staphylococcus aureus or enterococci. PCR-LDR exhibited a sensitivity and specificity of ≥98% for all components except tetracycline resistance, which had a sensitivity of 94.7%. Rapid and sensitive detection of antimicrobial resistance and virulence genes could help guide therapy and appropriate infection control measures.