Expression of HIF-1α and Genes Involved in Glucose Metabolism Is Increased in Cervical Cancer and HPV-16-Positive Cell Lines

• Published in: Pathogens (Basel) Vol. 12; no. 1; p. 33
• Main Authors: Priego-Hernández, Víctor D, Arizmendi-Izazaga, Adán, Soto-Flores, Diana G, Santiago-Ramón, Norma, Feria-Valadez, Milagros D, Navarro-Tito, Napoleón, Jiménez-Wences, Hilda, Martínez-Carrillo, Dinorah N, Salmerón-Bárcenas, Eric G, Leyva-Vázquez, Marco A, Illades-Aguiar, Berenice, Alarcón-Romero, Luz Del C, Ortiz-Ortiz, Julio
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 25.12.2022; MDPI
• Subjects: Acidosis; Bioinformatics; Biopsy; Cancer; CD147 antigen; Cell cycle; Cervical cancer; Cervix; Correlation analysis; Datasets; DNA polymerase; Enzymes; Gene expression; Genes; Genital tract; Genomes; Glucose; Glucose metabolism; HIF-1α; HPV 16; Human papillomavirus; Hypoxia; Hypoxia-inducible factor 1a; Medical prognosis; metabolic reprogramming; Metabolism; Patients; Proteins; Risk analysis; Risk factors; Survival analysis; Thermal cycling; Tissue analysis; Tissues; Transcription factors; Tumor cell lines; United States > US; Grand Island New York; HIF-1α; HPV 16; cervical cancer; glucose metabolism; metabolic reprogramming
• ISSN: 2076-0817; 2076-0817
• DOI: 10.3390/pathogens12010033

Cervical cancer (CC) is the most common cancer in women in the lower genital tract. The main risk factor for developing CC is persistent infection with HPV 16. The E6 and E7 oncoproteins of HPV 16 have been related to metabolic reprogramming in cancer through the regulation of the expression and stability of HIF-1α and consequently of the expression of its target genes, such as (HIF-1α), (GLUT1), , (CAIX), (MCT4), and (Basigin or CD147), which are involved in glucose metabolism. This work aimed to evaluate the expression of HIF-1α, GLUT1, LDHA, CAIX, MCT4, and Basigin in patient samples and CC cell lines. To evaluate the expression level of , and genes in tissue from patients with CC and normal tissue, the TCGA dataset was used. To evaluate the expression level of these genes by RT-qPCR in CC cell lines, HPV-negative (C-33A) and HPV-16-positive (SiHa and Ca Ski) cell lines were used. Increased expression of , and was found in Ca Ski and in SiHa compared to C-33A. Similar results were observed in CC tissues compared to normal tissue obtained by bioinformatics analysis. In conclusion, the expression of HIF-1α, GLUT1, LDHA, CAIX, MCT4, and BSG genes is increased in CC and HPV-16-positive cell lines.