Optimization of Ultrasonic-Microwave Assisted Extraction and Hepatoprotective Activities of Polysaccharides from Trametes orientalis

• Published in: Molecules (Basel, Switzerland) Vol. 24; no. 1; p. 147
• Main Authors: Zheng, Yi, Cui, Jue, Chen, An-Hui, Zong, Zhi-Min, Wei, Xian-Yong
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 02.01.2019; MDPI
• Subjects: Alanine; Alanine transaminase; Alcohol; Alcohols; Animal models; Antioxidants; Aspartate aminotransferase; Catalase; Exposure; Glutathione peroxidase; hepatoprotective activity; Hepatotoxicity; IL-1β; Inflammation; Liver; liver injury; Malondialdehyde; Optimization; Oxidative stress; Peroxidase; Polysaccharides; Pretreatment; Response surface methodology; Saccharides; Spleen; Statistical analysis; Trametes orientalis; Tumor necrosis factor-α; ultrasonic-microwave assisted extraction; Variables; Variance analysis; polysaccharides; liver injury; hepatoprotective activity; response surface methodology; Trametes orientalis; ultrasonic-microwave assisted extraction
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules24010147

Ultrasonic-microwave assisted extraction (UMAE) of polysaccharides was optimized by response surface methodology. Hepatoprotective effects of a purified polysaccharide (TOP-2) were evaluated by alcohol-induced liver injury model mice. The optimal UMAE parameters were indicated as below: ratio of water to raw material 28 mL/g, microwave power 114 W, extraction time 11 min. The polysaccharides yield was 7.52 ± 0.12%, which was well consistent with the predicted value of 7.54%. Pre-treatment with TOP-2 effectively increased the liver index and spleen index in alcohol-treated mice. The elevated serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels of mice after alcohol exposure were inhibited by TOP-2 administration. The liver tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) levels have decreased significantly as a result of alcohol exposure, while pre-treatment with TOP-2 could mitigate these consequences. Furthermore, pre-treatment with TOP-2 could efficiently boost the superoxidase dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activities, and observably constrain the malondialdehyde (MDA) level. The findings suggest that TOP-2 might be useful for alleviating the alcohol-induced hepatotoxicity via its antioxidant and anti-inflammatory potential.