Prevalence, Risk Factors, and Molecular Detection of Campylobacter in Farmed Cattle of Selected Districts in Bangladesh

• Published in: Pathogens (Basel) Vol. 10; no. 3; p. 313
• Main Authors: Hoque, Nazmul, Islam, S K Shaheenur, Uddin, Md Nasir, Arif, Mohammad, Haque, A K M Ziaul, Neogi, Sucharit Basu, Hossain, Md Mehedi, Yamasaki, Shinji, Kabir, S M Lutful
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 07.03.2021; MDPI
• Subjects: Animals; Bacteria; Bacterial infections; Biosecurity; Campylobacter; Campylobacter spp; Cattle; Confidence intervals; Dairy cattle; Dairy farms; Disinfection; farmed cattle; Farms; Feces; Feeds; Fetuses; Food contamination; Hygiene; Infections; Manures; Pathogens; PCR; Polymerase chain reaction; Poultry; prevalence; Risk analysis; Risk factors; Seasons; sequencing; Statistical analysis; Statistical methods; Wash water; Water analysis; Water sampling; Wind; Zoonoses; Bangladesh; Campylobacter spp; prevalence; Bangladesh; risk factors; farmed cattle; PCR; sequencing
• ISSN: 2076-0817; 2076-0817
• DOI: 10.3390/pathogens10030313

A cross-sectional survey was conducted in selected districts of Bangladesh to estimate prevalence, risk factors, and molecular detection of isolates from 540 farmed cattle of 90 herds. As an individual sample, 540 feces, and as a pooled sample, 180 milk samples, 90 feed samples, 90 water samples, 90 manure samples, and 90 animal attendants' hand-rinse water were collected and tested via culture, biochemical, and molecular assays. A pretested semi-structured questionnaire was used to collect herd-level data on risk factors with the herd owners. The herd-level data on risk factors were analyzed through univariate and multivariate analyses, and a -value <0.05 was considered statistically significant for all analyses. Overall, farm-level prevalence of bovine was enumerated to be 53.3% (95% confidence interval [CI]: 42.5-63.9%). The feces sample was found to be a high level of contamination of 30.9% (95% CI: 27-35%) followed by the manure swab (pooled) at 15.6% (95% CI: 8.8-24.7%). was documented as an abundant species (12.6%), followed by (5.1%), and (0.3%). Older farms (>5 years of age), no/minimum cleaning and disinfection practices, along with animal roaming outside of the farm, were documented as significant risk factors for farm-level occurrence. Evidence-based control measures need to be taken through stringent biosecurity and hygienic measurement to lessen the load of the pathogen in the farm environment and prevent further transmission to animals and humans.