Anti-Apoptotic and Anti-Inflammatory Properties of Grapefruit IntegroPectin on Human Microglial HMC3 Cell Line

In this study, we investigated the beneficial effects of grapefruit IntegroPectin, derived from industrial waste grapefruit peels via hydrodynamic cavitation, on microglia cells exposed to oxidative stress conditions. Grapefruit IntegroPectin fully counteracted cell death and the apoptotic process i...

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Published inCells (Basel, Switzerland) Vol. 13; no. 4; p. 355
Main Authors Scordino, Miriana, Urone, Giulia, Frinchi, Monica, Valenza, Chiara, Bonura, Angela, Cipollina, Chiara, Ciriminna, Rosaria, Meneguzzo, Francesco, Pagliaro, Mario, Mudò, Giuseppa, Di Liberto, Valentina
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 01.02.2024
Subjects
1-Phosphatidylinositol 3-kinase
Akt
AKT protein
Anti-inflammatory agents
Apoptosis
Brain research
Butyl hydroperoxide
By-products
Cavitation
Cell culture
Cell death
Citrus fruits
Control
Degeneration
ERK
Experiments
Grapefruit
Inflammation
Kinases
Lymphocytes B
Microglia
Morphology
Nervous system
Neurodegeneration
Neurodegenerative diseases
neuroinflammation
NF-κB protein
Nitric-oxide synthase
Oxidative stress
pectin
Physiological aspects
Proteins
Reactive oxygen species
Italy
United States > US
Germany
neuroinflammation
NF-kB
pectin
iNOS
Akt
microglia
oxidative stress
ERK
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Summary:In this study, we investigated the beneficial effects of grapefruit IntegroPectin, derived from industrial waste grapefruit peels via hydrodynamic cavitation, on microglia cells exposed to oxidative stress conditions. Grapefruit IntegroPectin fully counteracted cell death and the apoptotic process induced by cell exposure to tert-butyl hydroperoxide (TBH), a powerful hydroperoxide. The protective effects of the grapefruit IntegroPectin were accompanied with a decrease in the amount of ROS, and were strictly dependent on the activation of the phosphoinositide 3-kinase (PI3K)/Akt cascade. Finally, IntegroPectin treatment inhibited the neuroinflammatory response and the basal microglia activation by down-regulating the PI3K- nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB)- inducible nitric oxide synthase (iNOS) cascade. These data strongly support further investigations aimed at exploring IntegroPectin's therapeutic role in in vivo models of neurodegenerative disorders, characterized by a combination of chronic neurodegeneration, oxidative stress and neuroinflammation.
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ISSN:2073-4409
2073-4409
DOI:10.3390/cells13040355