Astilbin Inhibits the Activity of Sortase A from Streptococcus mutans
( ) is the primary etiological agent of dental caries. The enzyme sortase A (SrtA) is responsible for anchoring bacterial cell wall surface proteins involved in host cell attachment and biofilm formation. Thus, SrtA is an attractive target for inhibiting dental caries caused by -associated acid ferm...
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Published in | Molecules (Basel, Switzerland) Vol. 24; no. 3; p. 465 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
MDPI AG
28.01.2019
MDPI |
Subjects | |
Online Access | Get full text |
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Summary: | (
) is the primary etiological agent of dental caries. The
enzyme sortase A (SrtA) is responsible for anchoring bacterial cell wall surface proteins involved in host cell attachment and biofilm formation. Thus, SrtA is an attractive target for inhibiting dental caries caused by
-associated acid fermentation. In this study, we observed that astilbin, a flavanone compound extracted from
, has potent inhibitory activity against the
SrtA, with an IC
of 7.5 μg/mL. In addition, astilbin was proven to reduce the formation of biofilm while without affecting the growth of
. The results of a molecular dynamics simulation and a mutation analysis revealed that the Arg213, Leu111, and Leu116 of SrtA are important for the interaction between SrtA and astilbin. The results of this study demonstrate the potential of using astilbin as a nonbactericidal agent to modulate pathogenicity of
by inhibiting the activity of SrtA. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1420-3049 1420-3049 |
DOI: | 10.3390/molecules24030465 |