Scanning and non-scanning surface plasmon microscopy to observe cell adhesion sites

We observe adhesion sites of a cell on a substrate with high resolution. Since this observation requires interfacial measurements between the cell and the substrate, we employ scanning localized surface plasmon microscopy. We experimentally show that focal adhesion sites of a mouse muscle cell can b...

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Published inBiomedical optics express Vol. 3; no. 2; pp. 354 - 359
Main Authors Watanabe, Koyo, Matsuura, Koji, Kawata, Fukukazu, Nagata, Kotaro, Ning, Jun, Kano, Hiroshi
Format Journal Article
LanguageEnglish
Published United States Optical Society of America 01.02.2012
Subjects
Cell Studies
(170.1530) Cell analysis
(240.6680) Surface plasmons
(180.4243) Near-field microscopy
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Summary:We observe adhesion sites of a cell on a substrate with high resolution. Since this observation requires interfacial measurements between the cell and the substrate, we employ scanning localized surface plasmon microscopy. We experimentally show that focal adhesion sites of a mouse muscle cell can be observed without fluorescent labeling. We also show that a non-scanning surface plasmon microscope combined with the scanning localized surface plasmon microscope contributes to observing an entire cell adhesion site and identify regions of interest.
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ISSN:2156-7085
2156-7085
DOI:10.1364/boe.3.000354