Animal Pathogen-Like Type III Secretion System Is Required for the Intracellular Survival of Burkholderia mallei within J774.2 Macrophages
Burkholderia mallei is a highly infectious gram-negative pathogen and is the causative agent of human and animal glanders. By generating polar mutations (disruption of bsaQ and bsaZ) in the B. mallei ATCC 23344 animal pathogen-like type III secretion system (TTS), we demonstrate that this bacterial...
Saved in:
Published in | Infection and Immunity Vol. 74; no. 7; pp. 4349 - 4353 |
---|---|
Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Washington, DC
American Society for Microbiology
01.07.2006
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Burkholderia mallei is a highly infectious gram-negative pathogen and is the causative agent of human and animal glanders. By generating polar mutations (disruption of bsaQ and bsaZ) in the B. mallei ATCC 23344 animal pathogen-like type III secretion system (TTS), we demonstrate that this bacterial protein delivery system is required for intracellular growth of B. mallei in J774.2 cells, formation of macrophage membrane protrusions, actin polymerization, and phagosomal escape. These findings suggest that TTS plays a role in the intracellular trafficking of B. mallei and may facilitate cell-to-cell spread via actin-based motility. |
---|---|
Bibliography: | http://iai.asm.org/ ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Editor: V. J. DiRita Corresponding author. Mailing address: Bacteriology Division, USAMRIID, 1425 Porter St., Fort Detrick, Frederick, MD 21702. Phone: (301) 619-8332. Fax: (301) 619-8351. E-mail: Ricky.Ulrich@AMEDD.ARMY.MIL. |
ISSN: | 0019-9567 1098-5522 |
DOI: | 10.1128/IAI.01939-05 |