Purification and Structural Analysis of the Effective Anti-TMV Compound ε-Poly-l-lysine Produced by Streptomyces ahygroscopicus

• Published in: Molecules (Basel, Switzerland) Vol. 24; no. 6; p. 1156
• Main Authors: Chen, Jianguang, Liu, He, Xia, Zihao, Zhao, Xiuxiang, Wu, Yuanhua, An, Mengnan
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 23.03.2019; MDPI
• Subjects: Actinomycetes; Amberlite (trademark); anti-TMV activity; Antiviral activity; Biochemical characteristics; Biochemistry; Carbon; Chromatography; Column chromatography; Fermentation; Infrared analysis; Infrared lasers; Ion exchange; Ion exchange resins; Ionization; isolation and purification; Lysine; Maximum likelihood method; Metabolites; Microorganisms; Molecular weight; Morphology; Mountains; Natural products; NMR; NMR spectroscopy; Nuclear magnetic resonance; Pathogenesis; Pesticides; Phylogenetics; Phylogeny; Physical characteristics; Physiology; Plant diseases; Plant viruses; Poly-L-lysine; Polymerization; Proteins; rRNA 16S; Secondary metabolites; Spectrum analysis; Streptomyces; Streptomyces ahygroscopicus; Structural analysis; structural determination; Thin layer chromatography; Tobacco; Viruses; ε-poly- l -lysine; Liaoning China; China; isolation and purification; Streptomyces ahygroscopicus; structural determination; ε-poly-l-lysine; anti-TMV activity
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules24061156

Microbial secondary metabolites produced by actinomycetes are important natural products widely applied to control plant diseases. A variety of actinomycetes were isolated from soil samples collected from Tianzhu Mountain in Shenyang, China. A strain Shenyang Tianzhu (STZ) exhibits effective antiviral activity against Tobacco mosaic virus (TMV). The isolate was identified as based on its cultural, morphological, physiological, biochemical characteristics as well as the phylogenetic analysis using 16S rRNA sequences. To obtain the pure anti-TMV compound from STZ, the culture broth was subjected to Amberlite IRC-50 ion-exchange resin, SX-8 macroporous adsorption resin and Sephadex G-25 gel column chromatography. The purified active compound was confirmed to be ε-poly-l-lysine (ε-PL), with molecular mass in the range of 3454⁻4352 Da by structural analysis with infrared (IR), matrix-assisted laser desorption ionization-time-of-flight MS (MALDI-TOF), thin-layer chromatography (TLC) and high-resolution magic angle spinning nuclear magnetic resonance (HR-MAS NMR). The protective and curative effects of the purified compound ε-PL were tested and the results showed that the compound exhibited significant protective and curative activity against TMV. The potential application of ε-PL as an efficient anti-plant virus agent was expected.