An Ultra-High Performance Liquid Chromatography with Tandem Mass Spectrometry Method for Determination of 10 Alkaloids in Beagle Dog Plasma after the Oral Administration of the Corydalis yanhusuo W.T. Wang Extract and Yuanhuzhitong Tablets

• Published in: Molecules (Basel, Switzerland) Vol. 23; no. 8; p. 1925
• Main Authors: Cui, Binbin, Yang, Jing, Wang, Zhibin, Wu, Chengcui, Dong, Hongrui, Ren, Yixuan, Yang, Chunjuan
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 02.08.2018; MDPI
• Subjects: Acetonitrile; Administration, Oral; Alkaloids; Alkaloids - administration & dosage; Alkaloids - analysis; Alkaloids - blood; Alkaloids - chemistry; Analytical chemistry; Animals; Berberine; Calibration; Chromatography; Chromatography, High Pressure Liquid; Corydalis - chemistry; Corydalis yanhusuo; Corydalis yanhusuo W.T. Wang; Dogs; Drugs, Chinese Herbal - chemistry; Electrospraying; Elution; Flow velocity; Formic acid; High performance liquid chromatography; Ionization; Ions; Limit of Detection; Liquid chromatography; Mass spectrometry; Mass spectroscopy; Oral administration; pharmacokinetics; Pharmacology; Plant Extracts - chemistry; Regression Analysis; Scientific imaging; Spectrometry, Mass, Electrospray Ionization; Spectroscopy; Tablets; Tandem Mass Spectrometry - methods; ultra-high performance liquid chromatography-electrospray ionization-tandem mass spectrometry; Yuanhuzhitong tablets; pharmacokinetics; ultra-high performance liquid chromatography-electrospray ionization-tandem mass spectrometry; Corydalis yanhusuo W.T. Wang; Yuanhuzhitong tablets
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules23081925

This study has developed a sensitive and simple ultra-high performance liquid chromatography-electrospray ionization-tandem mass spectrometry method for the simultaneous determination of corydaline, dehydrocorydaline, tetrahydropalmatine, protopine, palmatine, tetrahydroberberine, columbamine, berberine, coptisine and berberrubine in beagle dog plasma after the oral administration of the W.T. Wang and Yuanhuzhitong tablets. Chromatographic separation was achieved on an Agilent Eclipse Plus C18 RRHD column (1.8 µm, 50 × 2.1 mm) using a gradient elution program with a mobile phase consisting of acetonitrile and water containing 0.1% formic acid at a flow rate of 0.3 mL/min. A tandem mass spectrometric detection was conducted by multiple reaction monitoring (MRM) mode via an electrospray ionization source in the positive mode. The calibration curves of all analytes showed good linear ( ² > 0.9800). The intra-day and inter-day precisions were less than 15% and the accuracies were within ±15%. The extraction recoveries conformed to the acceptable range. And there was no interference of endogenous substances in the sensitive assay method. All analytes were proven to be stable during sample storage and analysis procedures. The pharmacokinetic study indicated that the Yuanhuzhitong tablets could get a better absorption than W.T. Wang.