Isotope-Labeled RNA Building Blocks for NMR Structure and Dynamics Studies

RNA structural research lags behind that of proteins, preventing a robust understanding of RNA functions. NMR spectroscopy is an apt technique for probing the structures and dynamics of RNA molecules in solution at atomic resolution. Still, RNA analysis by NMR suffers from spectral overlap and line...

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Published inMolecules (Basel, Switzerland) Vol. 26; no. 18; p. 5581
Main Authors Olenginski, Lukasz T, Taiwo, Kehinde M, LeBlanc, Regan M, Dayie, Theodore K
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 14.09.2021
MDPI
Subjects
Biomass
Biosynthesis
Costs
Dynamic structural analysis
E coli
Enzymes
Glycerol
Isotope Labeling - methods
Line broadening
Line spectra
Magnetic resonance spectroscopy
Magnetic Resonance Spectroscopy - methods
Methods
NMR
NMR spectroscopy
Nuclear magnetic resonance
Nuclear Magnetic Resonance, Biomolecular - methods
Nucleic Acid Conformation
nucleotides
Proteins
Review
RNA
RNA - chemistry
RNA polymerase
stable isotope
Stable isotopes
structure
dynamics
RNA
enzymes
stable isotope
NMR spectroscopy
nucleotides
structure
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Summary:RNA structural research lags behind that of proteins, preventing a robust understanding of RNA functions. NMR spectroscopy is an apt technique for probing the structures and dynamics of RNA molecules in solution at atomic resolution. Still, RNA analysis by NMR suffers from spectral overlap and line broadening, both of which worsen for larger RNAs. Incorporation of stable isotope labels into RNA has provided several solutions to these challenges. In this review, we summarize the benefits and limitations of various methods used to obtain isotope-labeled RNA building blocks and how they are used to prepare isotope-labeled RNA for NMR structure and dynamics studies.
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These authors have contributed equally to this work.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules26185581