Arenaviral Nucleoproteins Suppress PACT-Induced Augmentation of RIG-I Function To Inhibit Type I Interferon Production

• Published in: Journal of virology Vol. 92; no. 13
• Main Authors: Shao, Junjie, Huang, Qinfeng, Liu, Xiaoying, Di, Da, Liang, Yuying, Ly, Hinh
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 01.07.2018
• Subjects: Arenaviridae Infections - genetics; Arenaviridae Infections - metabolism; Arenaviridae Infections - virology; Arenavirus - pathogenicity; HEK293 Cells; Host-Pathogen Interactions; Humans; Interferon Type I - metabolism; Nucleoproteins - genetics; Nucleoproteins - metabolism; Pathogenesis and Immunity; Pichinde virus - physiology; Receptors, Retinoic Acid - genetics; Receptors, Retinoic Acid - metabolism; RNA-Binding Proteins - genetics; RNA-Binding Proteins - metabolism; Viral Proteins - genetics; Viral Proteins - metabolism; Virus Replication; arenaviruses; type I interferon; PACT; nucleoprotein; RIG-I; Lassa virus; RNase activity
• ISSN: 0022-538X; 1098-5514
• DOI: 10.1128/jvi.00482-18

RIG-I is a major cytoplasmic sensor of viral pathogen-associated molecular pattern (PAMP) RNA and induces type I interferon (IFN) production upon viral infection. A double-stranded RNA (dsRNA)-binding protein, PACT, plays an important role in potentiating RIG-I function. We have shown previously that arenaviral nucleoproteins (NPs) suppress type I IFN production via their RNase activity to degrade PAMP RNA. We report here that NPs of arenaviruses block the PACT-induced enhancement of RIG-I function to mediate type I IFN production and that this inhibition is dependent on the RNase function of NPs, which is different from that of a known mechanism of other viral proteins to abolish the interaction between PACT and RIG-I. To understand the biological roles of PACT and RIG-I in authentic arenavirus infection, we analyze growth kinetics of recombinant Pichinde virus (PICV), a prototypical arenavirus, in RIG-I knockout (KO) and PACT KO mouse embryonic fibroblast (MEF) cells. Wild-type (WT) PICV grew at higher titers in both KO MEF lines than in normal MEFs, suggesting the important roles of these cellular proteins in restricting virus replication. PICV carrying the NP RNase catalytically inactive mutation could not grow in normal MEFs but could replicate to some extent in both KO MEF lines. The level of virus growth was inversely correlated with the amount of type I IFNs produced. These results suggest that PACT plays an important role in potentiating RIG-I function to produce type I IFNs in order to restrict arenavirus replication and that viral NP RNase activity is essential for optimal viral replication by suppressing PACT-induced RIG-I activation. We report here a new role of the nucleoproteins of arenaviruses that can block type I IFN production via their specific inhibition of the cellular protein sensors of virus infection (RIG-I and PACT). Our results suggest that PACT plays an important role in potentiating RIG-I function to produce type I IFNs in order to restrict arenavirus replication. This new knowledge can be exploited for the development of novel antiviral treatments and/or vaccines against some arenaviruses that can cause severe and lethal hemorrhagic fever diseases in humans.