Antibacterial activity of the stem bark of Tieghemella Heckelii Pierre ex. A Chev against methicillin-resistant Staphylococcus aureus

• Published in: BMC complementary and alternative medicine Vol. 17; no. 1; p. 170
• Main Authors: Kipre, B G, Guessennd, N K, Koné, M W, Gbonon, V, Coulibaly, J K, Dosso, M
• Format: Journal Article
• Language: English
• Published: England BioMed Central 27.03.2017; BMC
• Subjects: Agar; Amides; Anti-Bacterial Agents - isolation & purification; Anti-Bacterial Agents - pharmacology; Antibacterial; Antibacterial activity; Antibiotic resistance; Antibiotics; Antibodies; Antimicrobial activity; Antimicrobial agents; Assaying; Bacteria; Bactericidal activity; Bark; Bioassays; Chains; Chemical analysis; Collection; Concentration (composition); Cotton; Côte d’Ivoire; Diagnosis; Dilution; Drug resistance; Drugs; Ethanol; Ethyl acetate; Flora; Food; Food plants; Fractionation; Fungi; Gene mapping; Herbal medicine; Hospitals; Inactivation; Infectious diseases; Inhibition; Inoculum; Medicinal plants; Medicine; Methicillin - pharmacology; Methicillin-resistant Staphylococcus aureus; Methicillin-Resistant Staphylococcus aureus - drug effects; Methicillin-Resistant Staphylococcus aureus - growth & development; Microbial Sensitivity Tests; Minimum inhibitory concentration; Molecular structure; Multidrug resistance; Nosocomial infection; Nosocomial infections; Pharmaceutical industry; Pharmacokinetics; Pharmacology; Plant Bark - chemistry; Plant extracts; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Plants; Polarity; Populations; Public health; Sapotaceae - chemistry; Solvents; Staphylococcus aureus; Staphylococcus aureus - drug effects; Staphylococcus aureus - physiology; Staphylococcus infections; Tieghemella heckelii; Variance analysis; Sub-Saharan Africa; Antibacterial; Methicillin-resistant Staphylococcus aureus; Côte d’Ivoire; Tieghemella heckelii
• ISSN: 1472-6882; 1472-6882
• DOI: 10.1186/s12906-017-1681-8

Tieghemella heckelii (Sapotaceae) is a medicinal plant used in Africa, particularly in Côte d'Ivoire for treating various diseases including infections. Identification of prospective antibacterial compounds from stem bark of this plant as a result of its medicinal virtue, led to screening activity against methicillin resistant bacteria. Six extracts (hexane, chloroform, ethyl acetate, ethanol, methanol and sterile distilled water) were prepared and tested on methicillin resistant Staphylococcus aureus (MRSA) using broth microdilution method for activity assessment. From this experiment, the minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) of the plant extracts were determined in sterile 96-well microplates in order to search for both bacteriostatic and bactericidal effects. Afterwards, data analysis was performed using GraphPad Prism5 software (One-way ANOVA and Turkey Multiple Comparison test). The results were then presented as Mean ± SD for experiment repeated three times. Four extracts (ethyl acetate, methanol, ethanol and sterile distilled water) showed credible potency, with strong, significant, and moderate growth inhibition of the MRSA tested. The MIC values which varied from 45 μg/mL to 97 μg/mL according to microbial phenotype, resolutely established the activity of the plant extracts. Additionally, the MBC values which varied, depending on the type of bacteria strain, revealed the bacteriostatic and bactericidal effects of the active extracts against Methicillin-resistant Staphylococcus aureus. The present study is a confirmation of the therapeutic potential of Tieghemella heckelii and its promising contribution to the discovery of a novel antibacterial drug pertaining to these resistant strains.