Evaluation of the AdvanDx VRE EVIGENE Assay for Detection of vanA in Vancomycin-Resistant Staphylococcus aureus

AdvanDx VRE EVIGENE, a commercial vanA/vanB DNA hybridization assay to identify vancomycin-resistant enterococci (VRE), was evaluated for the detection of vanA in Staphylococcus aureus. Performance was assessed using S. aureus, VRE, and vancomycin-intermediate and -susceptible isolates. The assay de...

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Published inJournal of Clinical Microbiology Vol. 45; no. 5; pp. 1611 - 1613
Main Authors Fosheim, G.E, Carey, R.B, Limbago, B.M
Format Journal Article
LanguageEnglish
Published Washington, DC American Society for Microbiology 01.05.2007
Subjects
Bacterial Proteins - analysis
Bacterial Proteins - genetics
Bacteriology
Biological and medical sciences
Carbon-Oxygen Ligases - analysis
Carbon-Oxygen Ligases - genetics
DNA, Bacterial
Fundamental and applied biological sciences. Psychology
Infectious diseases
Medical sciences
Microbial Sensitivity Tests
Microbiology
Miscellaneous
Nucleic Acid Hybridization - methods
Sensitivity and Specificity
Staphylococcus aureus
Staphylococcus aureus - drug effects
Staphylococcus aureus - genetics
Vancomycin Resistance
Enterococcus
Resistance
Streptococcaceae
Antibiotic
Microbiology
Bacteria
Micrococcales
Micrococcaceae
Vancomycin
Antibacterial agent
Detection
Staphylococcus aureus
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Summary:AdvanDx VRE EVIGENE, a commercial vanA/vanB DNA hybridization assay to identify vancomycin-resistant enterococci (VRE), was evaluated for the detection of vanA in Staphylococcus aureus. Performance was assessed using S. aureus, VRE, and vancomycin-intermediate and -susceptible isolates. The assay demonstrated 100% sensitivity and specificity when analyzed visually and by optical density.
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Corresponding author. Mailing address: Centers for Disease Control and Prevention, MS C16, 1600 Clifton Rd., Atlanta, GA 30333. Phone: (404) 639-2162. Fax: (404) 639-3822. E-mail: BLimbago@cdc.gov
ISSN:0095-1137
1098-660X
1098-5530
DOI:10.1128/JCM.02556-06