Hepatoprotective Constituents of Total Dibenzocyclooctadiene Lignans from Schisandra chinensis Based on the Spectrum-Effect Relationship

• Published in: Molecules (Basel, Switzerland) Vol. 26; no. 21; p. 6554
• Main Authors: Deng, Lu-Lu, Xie, Xu-Dong, Li, Jiang, Wang, Dao-Ping, Hao, Xiao-Jiang, Chen, Gang, Mu, Shu-Zhen
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 29.10.2021; MDPI
• Subjects: Animals; Biological activity; Biological effects; Carbon Tetrachloride; Cell Survival - drug effects; Cells, Cultured; Chemical activity; Chemical separation; Chinese medicine; Chromatography; Cluster Analysis; Constituents; Cyclooctanes - chemistry; Cyclooctanes - isolation & purification; Cyclooctanes - pharmacology; Drug dosages; Fingerprints; hepatoprotective effects; Herbal medicine; High performance liquid chromatography; high-performance liquid chromatography (HPLC) fingerprint; isolation and purification; Least-Squares Analysis; Lignans; Lignans - chemistry; Lignans - isolation & purification; Lignans - pharmacology; Liquid chromatography; Liver; Liver - drug effects; Mice; Molecular Structure; Natural products; Protective Agents - chemistry; Protective Agents - isolation & purification; Protective Agents - pharmacology; Quality control; R&D; Research & development; Retention; Schisandra - chemistry; Schisandra chinensis; Software; spectrum-effect relationship; Synergistic effect; Technology assessment; total dibenzocyclooctadiene lignans (TDL); isolation and purification; spectrum-effect relationship; total dibenzocyclooctadiene lignans (TDL); Schisandra chinensis; high-performance liquid chromatography (HPLC) fingerprint; hepatoprotective effects
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules26216554

To scientifically clarify the hepatoprotective constituents of Fructus Schizandrae chinensis, eleven batches samples of total dibenzocyclooctadiene lignans (TDL) from were prepared by using the optimum extraction technique. Characteristic high-performance liquid chromatography (HPLC) chromatograms were obtained through HPLC analysis technology, and the hepatoprotective effects of the eleven batches of TDL were evaluated by MTT assay. Based on the chemical and biological activity results, the spectrum-effect relationship between the characteristic HPLC fingerprints and the hepatoprotective effect of TDL was established using Minitab 16.0 data analysis software. On the basis of the spectrum-effect relationship, thirteen compounds ( - ) were obtained from the TDL by chemical natural product chemical separation and purification technology, and their structures were identified on the basis of the spectral data and the literature. Based on these compounds, thirteen common peaks among the thirty-three chromatographic peaks in the above HPLC fingerprints were identified. Our findings showed that some components, including, schisandrin B ( ), schisandrin A ( ), and schisandrol B ( ) had significant roles in promoting hepatoprotective activity. Preliminary verification of the spectrum-effect relationship of TDL from was carried out, and the results confirmed that the activity of a composite of these three key components in optimal ratios was better than that of any individual compound, which potentially confirmed the reliability of the spectrum-effect relationship and the synergistic effects of traditional Chinese medicine.