Protective Effects of L-Theanine on IPEC-J2 Cells Growth Inhibition Induced by Dextran Sulfate Sodium via p53 Signaling Pathway

• Published in: Molecules (Basel, Switzerland) Vol. 26; no. 22; p. 7002
• Main Authors: Zhang, Longlin, Ma, Mengmeng, Li, Zhengyi, Zhang, Haihan, He, Xi, Song, Zehe
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 19.11.2021; MDPI
• Subjects: Amino acids; Animal models; Animals; Apoptosis; Beverages; Biomarkers; Cdc2 protein; Cell culture; Cell cycle; Cell Cycle - drug effects; Cell division; Cell growth; Cell proliferation; Dextran; Dextran sulfate; Dextran Sulfate - pharmacology; Dextrans; Enterocytes - metabolism; Fibroblast growth factor 2; Food additives; Gene expression; Glutamates - pharmacology; Growth factors; Growth Inhibitors - pharmacology; Inflammation; IPEC-J2 cells; L-theanine; Metabolic disorders; Metabolism; Metabolites; Metabolomics; Molecular modelling; Morphology; Nucleotides; p53 Protein; proliferation; Signal transduction; Signal Transduction - drug effects; Small intestine; Sodium; Sulfates; Swine; Tea; Theanine; Trends; Tumor Suppressor Protein p53 - metabolism; Weaning; IPEC-J2 cells; L-theanine; proliferation; metabolomics
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules26227002

L-theanine is a nonprotein amino acid found in tea leaves and has been widely used as a safe food additive in beverages or foods because of its varied bioactivities. The aim of this study was to reveal the in vitro gastrointestinal protective effects of L-theanine in DSS-induced intestinal porcine enterocyte (IPEC-J2) cell models using molecular and metabolic methods. Results showed that 2.5% dextran sulfate sodium (DSS) treatment inhibited the cell proliferation of IPEC-J2 and blocked the normal operation of the cell cycle, while L-theanine pretreatment significantly preserved these trends to exert protective effects. L-theanine pre-treatment also up-regulated the EGF, CDC2, FGF2, Rb genes and down-regulated p53, p21 proliferation-related mRNA expression in DSS-treated cells, in accompany with p53 signaling pathway inhibition. Meanwhile, metabolomics analysis revealed that L-theanine and DSS treated IPEC-J2 cells have different metabolomic profiles, with significant changes in the key metabolites involved in pyrimidine metabolism and amino acid metabolism, which play an important role in nucleotide metabolism. In summary, L-theanine has a beneficial protection in DSS-induced IPEC-J2 cells via promoting proliferation and regulating metabolism disorders.