Expression and RNA Interference of Ribosomal Protein L5 Gene in Nilaparvata lugens (Hemiptera: Delphacidae)

• Published in: Journal of insect science (Tucson, Ariz.) Vol. 17; no. 3
• Main Authors: Zhu, Jiajun, Hao, Peiying, Lu, Chaofeng, Ma, Yan, Feng, Yalin, Yu, Xiaoping
• Format: Journal Article
• Language: English
• Published: United States Oxford University Press 01.05.2017
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Fat Body - metabolism; Female; Gene Expression; Genetic aspects; Hemiptera - genetics; Hemiptera - growth & development; Hemiptera - metabolism; Insect Proteins - genetics; Insect Proteins - metabolism; Male; Observations; Open Reading Frames; Oryza; Ovary - growth & development; Ovary - metabolism; Physiological aspects; Ribosomal proteins; Ribosomal Proteins - genetics; Ribosomal Proteins - metabolism; RNA Interference; Sequence Analysis, DNA; gene; ovarian development; RNA interference; Nilaparvata lugens; NlRPL5 gene
• ISSN: 1536-2442; 1536-2442
• DOI: 10.1093/jisesa/iex047

The ribosomal proteins play important roles in the growth and development of organisms. This study aimed to explore the function of NlRPL5 (GenBank KX379234), a ribosomal protein L5 gene, in the brown planthopper Nilaparvata lugens. The open reading frame of NlRPL5 was cloned from N. lugens based on a previous transcriptome analysis. The results revealed that the open reading frame of NlRPL5 is of 900 bp, encoding 299 amino acid residues. The reverse transcription quantitative PCR results suggested that the expression of NlRPL5 gene was stronger in gravid females, but was relatively low in nymphs, males, and newly emerged females. The expression level of NlRPL5 in the ovary was about twofolds of that in the head, thorax, or fat body. RNAi of dsNlRPL5 resulted in a significant reduction of mRNA levels, ∼50% decrease in comparison with the dsGFP control at day 6. Treatment of dsNlRPL5 significantly restricted the ovarian development, and decreased the number of eggs laid on the rice (Oryza sativa) plants. This study provided a new clue for further study on the function and regulation mechanism of NlRPL5 in N. lugens.