Cell responses to xenobiotics: Comparison of MCF7 multi-drug- and mussel blood cell multi-xenobiotic-defense mechanisms

• Published in: Marine environmental research Vol. 58; no. 2; pp. 209 - 213
• Main Authors: Marin, Matthieu, Legros, Hélène, Poret, Agnès, Leboulenger, François, Foll, Frank Le
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Oxford Elsevier Ltd 01.08.2004; Elsevier; Elsevier science
• Subjects: Analysis of Variance; Animal and plant ecology; Animal, plant and microbial ecology; Animals; ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism; Biological and medical sciences; Biological Transport - drug effects; Bivalvia; Bivalvia - metabolism; Calcein accumulation; Cell Behavior; Cell Line, Tumor; Cellular Biology; Colorimetry; Doxorubicin - pharmacology; Drug Resistance, Multiple - drug effects; Ecotoxicology; Fluoresceins - metabolism; Fluorometry; Fundamental and applied biological sciences. Psychology; Hemocytes - metabolism; Humans; Immunology; Innate immunity; Life Sciences; Marine; MCF7; MDR; MTT; Mussel blood cells; MXR; Mytilus edulis; Sea water ecosystems; Subcellular Processes; Synecology; Tetrazolium Salts; Thiazoles; Toxicology; Verapamil - pharmacology; Vincristine - pharmacology; Xenobiotics - pharmacology; Mussel blood cells; MDR; MCF7; MTT; MXR; Calcein accumulation; Marine environment; Drug; Xenobiotic
• ISSN: 0141-1136; 1879-0291
• DOI: 10.1016/j.marenvres.2004.03.016

Multi-drug resistance (MDR) in MCF7 breast cancer cells and multi-xenobiotic resistance (MXR) in mussel ( Mytilus edulis) blood cells (MBC) are well known mechanisms that contribute to the decrease in intracellular concentrations of many unrelated but cytotoxic compounds. In the present work, we have carried out comparative investigations of the MDR/MXR protective mechanisms using a rapid colorimetric assay for cell viability and calcein accumulation for MDR/MXR activities. These studies were performed using cultured MCF7 and MBC before and after in vitro exposure to xenobiotics. Our results indicate that a 5-day exposure to doxorubicin or vincristine decreased calcein accumulation in MBC which is consistent with an induction of multi-xenobiotic resistance. The increase in calcein accumulation provoked by 1-h treatment with 50 μM verapamil was much lower in MBC when compared to the P-glycoprotein overexpressing MCF7 cell line. We conclude that such microplate assays could be used in primary cultures of MBC to estimate the effects of various chemicals on MXR activity.