Fenofibrate Ameliorates Retinal Pigment Epithelium Injury Induced by Excessive Fat Through Upregulation of PI3K/AKT Signaling

• Published in: Drug design, development and therapy Vol. 17; pp. 3439 - 3452
• Main Authors: Wang, Xue, Liu, Xiaomei, Tzekov, Radouil, Yu, Chaofeng, Yang, Jiasong, Feng, Yuliang, Wu, Yajun, Xu, Yali, Li, Shiying, Li, Wensheng
• Format: Journal Article
• Language: English
• Published: New Zealand Dove Medical Press Limited 01.01.2023; Dove Medical Press
• Subjects: Animals; Apoptosis; Cytokines; Epithelium; Fenofibrate; Fenofibrate - pharmacology; high-fat diet (hfd); Inflammation; Mice; Oxidases; Oxidative Stress; palmitic acid (pa); Phosphatidylinositol 3-Kinases - metabolism; Proto-Oncogene Proteins c-akt - metabolism; Retinal Pigment Epithelium - metabolism; rpe; Saturated fatty acids; Signal Transduction; Tumor Necrosis Factor-alpha - metabolism; Up-Regulation; Vascular endothelial growth factor; Vascular Endothelial Growth Factor A - metabolism; China; fenofibrate; inflammation; palmitic acid; RPE; oxidative stress; high-fat diet
• ISSN: 1177-8881; 1177-8881
• DOI: 10.2147/DDDT.S420178

This study aimed to determine the effect and its mechanism of fenofibrate on retinal pigment epithelium (RPE) injury induced by excessive fat in vitro and in vivo. ARPE-19 cells were co-incubated with palmitic acid (PA) and fenofibric acid (the active form of fenofibrate after metabolism in vivo) and mice fed with high-fat diet (HFD) were supplemented with fenofibrate. The following methods were used: Western blot and immunofluorescent staining to determine expressions of reactive oxygen species (ROS)-associated factors and proinflammatory cytokines; electroretinogram (ERG) c-wave to evaluate RPE function; TUNEL staining to detect the apoptotic cell in RPE tissue. Additionally, ARPE19 cells were treated with PI3K/AKT inhibitor or agonist to investigate the mechanism of fenofibric acid inhibiting PA-induced RPE damage. We found that the application of PA inhibited RPE cell viability in a dose-dependent manner, and increased the levels of NAPDH oxidase 4 (NOX4), 3-nitrotyrosin (3-NT), intracellular adhesion molecule-1(ICAM1), tumor necrosis factor alpha (TNFα) and vascular endothelial growth factor (VEGF) at 400μM. The application of fenofibric acid resulted in the inhibition of NOX4, 3-NT, TNFα, ICAM1 and VEGF expression in ARPE-19 cells treated with PA. Moreover, wortmannin, as a selective inhibitor of PI3K/AKT pathway, abolished the effects of fenofibrate on the oxidative stress and inflammation in ARPE-19 cells. In addition, 740Y-P, a selective agonist of PI3K/AKT pathway, enhanced the protective action of fenofibrate. Meanwhile, in vivo dosing of fenofibrate ameliorated the downregulated amplitudes of ERG c-wave in HFD-fed mice and suppressed the HFD-induced oxidative injury and inflammatory response in RPE tissues. Our results suggested that fenofibrate ameliorated RPE cell damage induced by excessive fat in vitro and in vivo, in part, through activation of the PI3K/AKT signaling pathway.