The Role of Orientation of Surface Bound Dihydropyrrol-2-ones (DHP) on Biological Activity

• Published in: Molecules (Basel, Switzerland) Vol. 24; no. 14; p. 2676
• Main Authors: Taunk, Aditi, Chen, Renxun, Iskander, George, Ho, Kitty K K, Almohaywi, Basmah, Black, David StClair, Willcox, Mark D P, Kumar, Naresh
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 23.07.2019; MDPI
• Subjects: Acids; Anti-Bacterial Agents - chemical synthesis; Anti-Bacterial Agents - pharmacology; Antibacterial activity; Antibiotics; antimicrobial; Antimicrobial agents; Bacteria; Bacterial Adhesion - drug effects; Biofilms; Biofilms - drug effects; Biofilms - growth & development; Biological activity; biomaterials; Carbodiimide; Carbodiimides - chemistry; Carbon; Colonization; Contact angle; Cross-Linking Reagents - chemistry; Drug resistance; Ethyldimethylaminopropyl carbodiimide; Fluorine; Glass substrates; Infections; Infectious diseases; Medical equipment; Medical technology; Microbial Sensitivity Tests; Nitrogen; Photoelectron spectroscopy; Photoelectrons; Pseudomonas; Pseudomonas aeruginosa - drug effects; Pseudomonas aeruginosa - growth & development; Pyrrolidinones - chemical synthesis; Pyrrolidinones - pharmacology; Quorum sensing; Quorum Sensing - drug effects; Spectrum analysis; Staphylococcus; Staphylococcus aureus - drug effects; Staphylococcus aureus - growth & development; Structure-Activity Relationship; Substrates; Succinimides - chemistry; surface attachment; X ray photoelectron spectroscopy; United States > US; antimicrobial; quorum sensing; surface attachment; biomaterials; Pseudomonas; Staphylococcus
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules24142676

Quorum sensing (QS) signaling system is important for bacterial growth, adhesion, and biofilm formation resulting in numerous infectious diseases. Dihydropyrrol-2-ones (DHPs) represent a novel class of antimicrobial agents that inhibit QS, and are less prone to develop bacterial resistance due to their non-growth inhibition mechanism of action which does not cause survival pressure on bacteria. DHPs can prevent bacterial colonization and quorum sensing when covalently bound to substrates. In this study, the role of orientation of DHP compounds was investigated after covalent attachment by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC)/ -hydroxysuccinimide (NHS) coupling reaction to amine-functionalized glass surfaces via various positions of the DHP scaffold. The functionalized glass surfaces were characterized by X-ray photoelectron spectroscopy (XPS) and contact angle measurements and tested for their in vitro biological activity against and . DHPs attached via the N-1 position resulted in the highest antibacterial activities against , while no difference was observed for DHPs attached either via the N-1 position or the C-4 phenyl ring against