Deposition of Polymer Particles with Fibrinogen Corona at Abiotic Surfaces under Flow Conditions

• Published in: Molecules (Basel, Switzerland) Vol. 26; no. 20; p. 6299
• Main Authors: Żeliszewska, Paulina, Wasilewska, Monika, Cieśla, Michał, Adamczyk, Zbigniew
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 18.10.2021; MDPI
• Subjects: Adsorption; adsorption of fibrinogen; Aluminum Silicates - chemistry; Aluminum Silicates - metabolism; Colloids - chemistry; Colloids - metabolism; Coronas; deposition of particles with corona; Electrophoretic mobility; Experiments; Fibrinogen; Fibrinogen - chemistry; Fibrinogen - metabolism; fibrinogen corona at particles; Humans; Hydrogen-Ion Concentration; Immunoassays; Kinetics; Light scattering; Lysine; microfluidic impinging-jet cell; Osmolar Concentration; Photon correlation spectroscopy; Poly-L-lysine; Polymers; Polymers - chemistry; Polymers - metabolism; Proteins; Streaming potential; Substrate Specificity; Substrates; Surface Properties; Zeta potential; zeta potential of protein coronas; deposition of particles with corona; microfluidic impinging-jet cell; adsorption of fibrinogen; fibrinogen corona at particles; zeta potential of protein coronas
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules26206299

The deposition kinetics of polymer particles with fibrinogen molecule coronas at bare and poly-L-lysine (PLL) modified mica was studied using the microfluid impinging-jet cell. Basic physicochemical characteristics of fibrinogen and the particles were acquired using dynamic light scattering and the electrophoretic mobility methods, whereas the zeta potential of the substrates was determined using streaming potential measurements. Subsequently, an efficient method for the preparation of the particles with coronas, characterized by a controlled fibrinogen coverage, was developed. This enabled us to carry out measurements, which confirmed that the deposition kinetics of the particles at mica vanished at pH above 5. In contrast, the particle deposition of PLL modified mica was at maximum for pH above 5. It was shown that the deposition kinetics could be adequately analyzed in terms of the mean-field approach, analogously to the ordinary colloid particle behavior. This contrasts the fibrinogen molecule behavior, which efficiently adsorbs at negatively charged substrates for the entire range pHs up to 9.7. These results have practical significance for conducting label-free immunoassays governed by the specific antigen/antibody interactions.