Determination of Free Histidine in Complex Hair Care Products with Minimum Sample Preparation Using Cation-Exchange Chromatography and Post Column Derivatization

In this communication, we describe the first analytical method for the determination of free histidine in hair care products (shampoos and conditioners). Cation-exchange chromatography combined with postcolumn derivatization and fluorimetric detection enabled the accurate (recovery: 83.5-114.8%) and...

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Bibliographic Details
Published inMolecules (Basel, Switzerland) Vol. 28; no. 2; p. 888
Main Authors Tsiasioti, Apostolia, Zacharis, Constantinos K, Tzanavaras, Paraskevas D
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 16.01.2023
MDPI
Subjects
Cation exchanging
Cation-exchange chromatography
Chromatography
Chromatography, High Pressure Liquid - methods
Column chromatography
Communication
Copper
fluorescence
Fluorometry
Hair
hair care products
Hair conditioners
Hair Preparations
high-pressure liquid chromatography
Histidine
Humans
Indicators and Reagents
postcolumn derivatization
Reagents
Sample preparation
Shampoos
United States > US
Japan
postcolumn derivatization
hair care products
fluorescence
histidine
high-pressure liquid chromatography
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Summary:In this communication, we describe the first analytical method for the determination of free histidine in hair care products (shampoos and conditioners). Cation-exchange chromatography combined with postcolumn derivatization and fluorimetric detection enabled the accurate (recovery: 83.5-114.8%) and precise (2.4-5.6% RSD) determination of free histidine without matrix interferences at concentration levels down to 1.5 mg kg . Real commercially available samples were found to contain the amino acid at levels ranging between 70 and 535 mg kg .
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules28020888