Effects of the Species and Growth Stage on the Antioxidant and Antifungal Capacities, Polyphenol Contents, and Volatile Profiles of Bamboo Leaves

• Published in: Foods Vol. 13; no. 3; p. 480
• Main Authors: Shen, Hui, Wang, Yan, Shi, Pingping, Li, Hong, Chen, Yanan, Hu, Tenggen, Yu, Yuanshan, Wang, Jinxiang, Yang, Fang, Luo, Haibo, Yu, Lijuan
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.02.2024
• Subjects: Additives; Alcohols; Aldehydes; Aluminum; Antifungal agents; antifungal capacity; Antimicrobial agents; antioxidant capacity; Antioxidants; Bamboo; bamboo leaf; Bioactive compounds; Biological activity; Chromatography; Cluster analysis; Correlation coefficient; Correlation coefficients; Discriminant analysis; Esters; Ethanol; Flavonoids; Food additives; Food industry; Fruits; Fungi; Fungicides; Furans; Gas chromatography; genotype; Growth stage; Ionic mobility; Ketones; Leaves; Mass spectrometry; Organic compounds; Pesticides; Phenolic compounds; Phenols; Plant extracts; Polyphenols; Principal components analysis; Pyrazine; Reagents; Scavenging; Scientific imaging; Sodium; Spectrometry; Sulfonic acid; Terpenes; Vegetables; VOCs; Volatile compounds; Volatile organic compounds; volatile profile; Volatiles; China; bamboo leaf; antifungal capacity; volatile profile; antioxidant capacity; genotype
• ISSN: 2304-8158; 2304-8158
• DOI: 10.3390/foods13030480

Bamboo leaves contain high concentrations of various biologically active compounds, such as polyphenols and volatiles, making them attractive as raw resources for antioxidant additives in the food industry. Here, we investigated the total phenolic content (TPC) and total flavonoid content (TFC) of four bamboo leaf extracts from two species ( and ) at two growth stages (first and second years). Antioxidant capacity was determined based on the radical-scavenging capacity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS ). We also assessed the antifungal capacity based on mycelial growth inhibition of ( ), ( ), and ( ). Pearson's correlation coefficients showed that the TPC was significantly ( < 0.01) negatively correlated with the half-maximal inhibitory concentrations against DPPH and ABTS , whereas the TFC was positively correlated with and growth inhibition, which suggest that TPC and TFC might be the major contributors to the antioxidant and antifungal capacities of bamboo leaves, respectively. The volatile organic compounds (VOCs) of bamboo leaves were also analyzed using gas chromatography-ion mobility spectrometry. The VOCs included twenty-four aldehydes, eleven alcohols, four furans, seven esters, fifteen terpenes, three ketones, one pyrazine, and thirty unidentified compounds. Principal component analysis, partial least squares discriminant analysis, and hierarchical cluster analysis were performed to assess the differences in the volatile profiles of the four bamboo leaf samples, from which 23 discriminatory VOCs with variable importance in the projection values > 1 were screened, and part of them were impacted by species or growth stage. These findings provide a theoretical foundation for the use of bamboo leaves.