Protective Effects of Traditional Polyherbs on Cisplatin-Induced Acute Kidney Injury Cell Model by Inhibiting Oxidative Stress and MAPK Signaling Pathway

• Published in: Molecules (Basel, Switzerland) Vol. 25; no. 23; p. 5641
• Main Authors: Dachuri, VinayKumar, Song, Phil Hyun, Kim, Young Woo, Ku, Sae-Kwang, Song, Chang-Hyun
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 30.11.2020; MDPI
• Subjects: Acids; acute kidney injury; Acute Kidney Injury - chemically induced; Acute Kidney Injury - drug therapy; Acute Kidney Injury - metabolism; Acute Kidney Injury - pathology; acute renal failure; Animals; Annexin V; Antineoplastic Agents - toxicity; Antioxidants; Antioxidants - pharmacology; Apoptosis; Biological Products - pharmacology; Caspase-3; Catalase; Cell culture; Cell death; Cell growth; Cell injury; Cells, Cultured; Chemotherapy; Cisplatin; Cisplatin - toxicity; Free radicals; Gene Expression Regulation - drug effects; Humans; Kidney diseases; Kidneys; Kinases; MAP kinase; Medicine, Traditional; Mitogen-Activated Protein Kinases - metabolism; Mortality; nephroprotective; Oxidative stress; Oxidative Stress - drug effects; Plant Extracts - pharmacology; polyherb; Protective Agents - pharmacology; Protein kinase; Proteins; Rats; Reactive Oxygen Species - metabolism; renal cell injury; Renal failure; Renal function; Risk analysis; Risk factors; Scavenging; Signal transduction; Superoxide dismutase; Variance analysis; United States > US; renal cell injury; antioxidant; acute renal failure; antiapoptosis; polyherb; acute kidney injury; MAPK; cisplatin; nephroprotective
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules25235641

Acute kidney injury (AKI) is a disease caused by sudden renal dysfunction, which is an important risk factor for chronic renal failure. However, there is no effective treatment for renal impairment. Although some traditional polyherbs are commercially available for renal diseases, their effectiveness has not been reported. Therefore, we examined the nephroprotective effects of polyherbs and their relevant mechanisms in a cisplatin-induced cell injury model. Rat NRK-52E and human HK-2 subjected to cisplatin-induced AKI were treated with four polyherbs, Injinhotang (IJ), Ucha-Shinki-Hwan (US), Yukmijihwang-tang (YJ), and Urofen (Uro) similar with Yondansagan-tang, for three days. All polyherbs showed strong free radical scavenging activities, and the treatments prevented cisplatin-induced cell death in both models, especially at 1.2 mg/mL. The protective effects involved antioxidant effects by reducing reactive oxygen species and increasing the activities of superoxide dismutase and catalase. The polyherbs also reduced the number of annexin V-positive apoptotic cells and the expression of cleaved caspase-3, along with inhibited expression of mitogen-activated protein kinase-related proteins. These findings provide evidence for promoting the development of herbal formulas as an alternative therapy for treating AKI.