Simple, Accurate, Serotype-Specific PCR Assay To Differentiate Streptococcus pneumoniae Serotypes 6A, 6B, and 6C

In this study, we developed a simple, reliable, serotype-specific PCR method to differentiate Streptococcus pneumoniae serotypes 6A, 6B, and 6C. It was more efficient and practical than the assays currently being used to identify serotypes 6A, 6B, and 6C. Of 120 selected serogroup 6 isolates from su...

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Published inJournal of Clinical Microbiology Vol. 47; no. 8; pp. 2470 - 2474
Main Authors Jin, Ping, Xiao, Meng, Kong, Fanrong, Oftadeh, Shahin, Zhou, Fei, Liu, Chunyi, Gilbert, Gwendolyn L
Format Journal Article
LanguageEnglish
Published Washington, DC American Society for Microbiology 01.08.2009
American Society for Microbiology (ASM)
Subjects
Adolescent
Adult
Aged
Aged, 80 and over
Bacterial Typing Techniques - methods
Bacteriology
Base Sequence
Biological and medical sciences
Child
Child, Preschool
DNA Primers - genetics
Fundamental and applied biological sciences. Psychology
Genotype
Humans
Infant
Microbiology
Middle Aged
Miscellaneous
Molecular Sequence Data
New South Wales
Pneumococcal Infections - microbiology
Polymerase Chain Reaction - methods
Sensitivity and Specificity
Sequence Alignment
Serotyping
Streptococcus pneumoniae
Streptococcus pneumoniae - classification
Streptococcus pneumoniae - genetics
Streptococcus pneumoniae - isolation & purification
Young Adult
New South Wales
Polymerase chain reaction
Streptococcaceae
Microbiology
Bacteria
Micrococcales
Serotype
Streptococcus pneumoniae
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Summary:In this study, we developed a simple, reliable, serotype-specific PCR method to differentiate Streptococcus pneumoniae serotypes 6A, 6B, and 6C. It was more efficient and practical than the assays currently being used to identify serotypes 6A, 6B, and 6C. Of 120 selected serogroup 6 isolates from subjects with invasive (n = 101) and noninvasive (n = 19) pneumococcal disease, most of which were collected after 2003 in New South Wales, 45 had been identified as 6A and 75 had been identified as 6B by the Quellung reaction. PCR analysis confirmed the results for serotype 6B isolates and identified two different subtypes. Fourteen of 45 isolates that had been identified as serotype 6A actually belonged to serotype 6C.
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Corresponding author. Centre for Infectious Diseases and Microbiology (CIDM), Institute of Clinical Pathology and Medical Research (ICPMR), Westmead Hospital, Darcy Road, Westmead, New South Wales, 2145 Australia. Phone: (612) 9845 6255. Fax: (612) 9893 8659. E-mail: l.gilbert@usyd.edu.au
These authors contributed equally to the work.
ISSN:0095-1137
1098-660X
1098-660X
DOI:10.1128/JCM.00484-09