Antinociceptive Effect of Hinokinin and Kaurenoic Acid Isolated from Aristolochia odoratissima L

• Published in: Molecules (Basel, Switzerland) Vol. 25; no. 6; p. 1454
• Main Authors: Montiel-Ruiz, Rosa Mariana, Córdova-de la Cruz, Marcos, González-Cortázar, Manasés, Zamilpa, Alejandro, Gómez-Rivera, Abraham, López-Rodríguez, Ricardo, Lobato-García, Carlos Ernesto, Blé-González, Ever A
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 24.03.2020; MDPI
• Subjects: 4-Butyrolactone - analogs & derivatives; 4-Butyrolactone - chemistry; 4-Butyrolactone - therapeutic use; Acetic acid; Acids; Analgesics; Analgesics - chemistry; Analgesics - therapeutic use; Animals; Antidotes; antinociception; Aristolochia - chemistry; Aristolochia odoratissima; aristolochia odoratissima l; Benzodioxoles - chemistry; Benzodioxoles - therapeutic use; Chromatography, High Pressure Liquid; Column chromatography; Diterpenes - chemistry; Diterpenes - therapeutic use; Drug dosages; Ethyl acetate; Experiments; formalin test; High performance liquid chromatography; hinokinin; kaurenoic acid; Lignans - chemistry; Lignans - therapeutic use; Liquid chromatography; Magnetic Resonance Spectroscopy; Male; Medicine; Metabolites; Mice; Nonsteroidal anti-inflammatory drugs; Oils & fats; Pain; Pain - drug therapy; Pain Measurement; Pain perception; Phytochemicals; Plant extracts; Plant Extracts - chemistry; Plant Extracts - therapeutic use; Polarity; Mexico; kaurenoic acid; antinociception; hinokinin; Aristolochia odoratissima L; formalin test
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules25061454

L. is employed for the treatment of pain and as an antidote against the poison of venomous animals in traditional medicine. However, reports have not been found, to our knowledge, about the evaluation of the antinociceptive activity of extracts nor about the presence of compounds associated with this activity. Thus, the purpose of this work was to evaluate the antinociceptive activity of extracts and compounds isolated from the stems of L. The extracts were obtained with solvents of increasing polarity and the compounds were isolated and characterized by column chromatography, HPLC, and NMR. The antinociceptive activity was carried out by the formalin test in mice. Ethyl acetate (AoEA) and methanolic (AoM) extracts decreased the paw licking in both phases of the formalin test. The isolated compounds (kaurenoic acid and hinokinin) from AoEA showed the highest antinociceptive activity in both phases of the formalin test. These results confirmed the analgesic effect of this specie described in traditional medicine and provided a base for a novel analgesic agent. They also allowed an approach for the development of standardized plant extracts with isolated metabolites.