Ciprofloxacin-Resistant Salmonella enterica Serovar Typhi from Kuwait with Novel Mutations in gyrA and parC Genes

• Published in: Journal of Clinical Microbiology Vol. 47; no. 1; pp. 208 - 211
• Main Authors: Dimitrov, Tzonyo, Dashti, Ali A, Albaksami, Ossama, Udo, Edet E, Jadaon, Mehrez M, Albert, M. John
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01.01.2009; American Society for Microbiology (ASM)
• Subjects: Adult; Amino Acid Substitution - genetics; Anti-Bacterial Agents - pharmacology; Bacterial Proteins - genetics; Bacteriology; Biological and medical sciences; Blood - microbiology; Child, Preschool; Ciprofloxacin - pharmacology; DNA Gyrase - genetics; DNA Topoisomerase IV - genetics; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; Drug Resistance, Bacterial; Fundamental and applied biological sciences. Psychology; Humans; Kuwait; Male; Microbial Sensitivity Tests; Microbiology; Miscellaneous; Molecular Sequence Data; Mutation, Missense; Salmonella enterica; Salmonella typhi - drug effects; Salmonella typhi - genetics; Salmonella typhi - isolation & purification; Sequence Analysis, DNA; Typhoid Fever - microbiology; Kuwait; Asia; Resistance; Fluoroquinolone derivatives; Gene; Microbiology; Salmonella typhi; Bacteria; Mutation; Antibacterial agent; Ciprofloxacin; Quinolone derivatives; Enterobacteriaceae
• ISSN: 0095-1137; 1098-660X
• DOI: 10.1128/JCM.01161-08

Blood isolates of Salmonella enterica serovar Typhi from two recently returned Bangladeshi patients in Kuwait were ciprofloxacin resistant, with ciprofloxacin MICs of 12 mg/liter for both isolates. Both isolates had three novel gyrA mutations (55-Leu[rightward arrow]Trp, 87-Asp[rightward arrow]Ala, and 106-Gln[rightward arrow]Arg) and three novel parC mutations (84-Glu[rightward arrow]Lys, 106-Trp[rightward arrow]Gly, and 128-Tyr[rightward arrow]Asp).