Magnetic Porous Molecularly Imprinted Polymers Based on Surface Precipitation Polymerization and Mesoporous SiO₂ Layer as Sacrificial Support for Efficient and Selective Extraction and Determination of Chlorogenic Acid in Duzhong Brick Tea

• Published in: Molecules (Basel, Switzerland) Vol. 23; no. 7; p. 1554
• Main Authors: Peng, Mijun, Li, Huan, Long, Ruiqing, Shi, Shuyun, Zhou, Hanjun, Yang, Shuping
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 27.06.2018; MDPI
• Subjects: Chlorogenic acid; Chlorogenic Acid - chemistry; Chromatography, High Pressure Liquid; Computer applications; Computer simulation; Construction materials; Duzhong brick tea; Fabrication; High performance liquid chromatography; Imprinted polymers; Kinetics; Liquid chromatography; mesoporous SiO2; Molecular Imprinting - methods; Polymerization; Polymers; Polymers - chemistry; Precipitation; Reaction kinetics; Recognition; selective extraction; Silicon dioxide; Silicon Dioxide - chemistry; Solid phases; surface precipitation polymerization; Tea; Tea - chemistry; surface precipitation polymerization; mesoporous SiO2; chlorogenic acid; selective extraction; Duzhong brick tea
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules23071554

Magnetic porous molecularly imprinted polymers (MPMIPs) for rapid and efficient selective recognition of chlorogenic acid (CGA) were effectively prepared based on surface precipitation polymerization using CGA as template, 4-vinylpyridine (4-VP) as functional monomer, and mesoporous SiO₂ (mSiO₂) layer as sacrificial support. A computational simulation by evaluation of electronic binding energy is used to optimize the stoichiometric ratio between CGA and 4-VP (1:5), which reduced the duration of laboratory trials. The porous MIP shell and the rid of solid MIPs by magnet gave MPMIPs high binding capacity (42.22 mg/g) and fast kinetic binding (35 min). Adsorption behavior between CGA and MPMIPs followed Langmuir equation and pseudo-first-order reaction kinetics. Furthermore, the obtained MPMIPs as solid phase adsorbents coupled with high performance liquid chromatography (HPLC) were employed for selective extraction and determination of CGA (2.93 ± 0.11 mg/g) in Duzhong brick tea. The recoveries from 91.8% to 104.2%, and the limit of detection (LOD) at 0.8 μg/mL were obtained. The linear range (2.0⁻150.0 μg/mL) was wide with ² > 0.999. Overall, this study provided an efficient approach for fabrication of well-constructed MPMIPs for fast and selective recognition and determination of CGA from complex samples.