Identification of novel alpha- and gammaherpesviruses from cutaneous and mucosal lesions of dolphins and whales

• Published in: Journal of virological methods Vol. 136; no. 1; pp. 261 - 266
• Main Authors: Smolarek Benson, Kara A., Manire, Charles A., Ewing, Ruth Y., Saliki, Jeremiah T., Townsend, Forrest I., Ehlers, Bernhard, Romero, Carlos H.
• Format: Journal Article
• Language: English
• Published: London Elsevier B.V 01.09.2006; Amsterdam Elsevier; New York, NY
• Subjects: Alphaherpesvirinae; Amino Acid Sequence; Animals; Biological and medical sciences; Cetaceae; DNA, Viral - analysis; DNA, Viral - genetics; Dolphins - virology; Fundamental and applied biological sciences. Psychology; Gammaherpesvirinae; Grampus griseus; Herpes simplex virus; Herpesviridae - classification; Herpesviridae - genetics; Herpesviridae - isolation & purification; Herpesvirus; Kogia sima; Mesoplodon densirostris; Microbiology; Molecular identification; Molecular Sequence Data; Mucous Membrane - virology; Phylogeny; Polymerase Chain Reaction - methods; Sequence Analysis, DNA; Sequence Homology; Skin Diseases, Viral - veterinary; Techniques used in virology; Tursiops truncatus; Virology; Virus Diseases - veterinary; Whales - virology; Gammaherpesvirinae; Cetaceae; Alphaherpesvirinae; Molecular identification; Herpesvirus; Skin disease; Microbiology; Herpesviridae; Mucosa; Identification; Method; Virology; Virus

Total DNA extracted from mucosal and skin lesions of captive and stranded cetaceans was analyzed for herpesvirus DNA by nested and direct polymerase chain reactions (PCR). The targeted sequences corresponded to a region of the DNA polymerase gene containing multiple conserved amino acid motifs. Herp...