LC-ESI-MS/MS Identification of Biologically Active Phenolics in Different Extracts of Alchemilla acutiloba Opiz

• Published in: Molecules (Basel, Switzerland) Vol. 27; no. 3; p. 621
• Main Authors: Dos Santos Szewczyk, Katarzyna, Pietrzak, Wioleta, Klimek, Katarzyna, Grzywa-Celińska, Anna, Celiński, Rafał, Gogacz, Marek
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 18.01.2022; MDPI
• Subjects: Alchemilla; Alchemilla - chemistry; Animals; Anti-Inflammatory Agents - pharmacology; antioxidant; Antioxidants; Antioxidants - pharmacology; Biological activity; Blood vessels; Butanol; Chromatography, Liquid; Cytotoxicity; Cytotoxins - pharmacology; Flavonoids; Flavonoids - analysis; Flavonoids - pharmacology; Glucosides; Haplorhini; Inflammation; Ionization; Kaempferol; Kidney - drug effects; Kidney - pathology; LC-MS; Liquid chromatography; Naringenin; Phenolic acids; Phenols; Phenols - analysis; Plant Extracts - analysis; Plant Extracts - pharmacology; Polyphenols; Qualitative analysis; Quantitative analysis; Rosaceae; Spectrometry; Spectrometry, Mass, Electrospray Ionization; Tandem Mass Spectrometry; LC-MS; antioxidant; Alchemilla; cytotoxicity; Rosaceae
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules27030621

Liquid chromatography electrospray ionization tandem mass spectrometric (LC-ESI-MS/MS) qualitative and quantitative analysis of different extracts from the aerial parts and roots of led to the identification of phenolic acids and flavonoids. To the best of our knowledge, isorhamnetin 3-glucoside, kaempferol 3-rutinoside, narcissoside, naringenin 7-glucoside, 3- -methylquercetin, naringenin, eriodictyol, rhamnetin, and isorhamnetin were described for the first time in genus. In addition, the antioxidant, anti-inflammatory and cytotoxic activity of all extracts were evaluated. The results clearly showed that among analyzed extracts, the butanol extract of the aerial parts exhibited the highest biological activity comparable with the positive controls used.