Modification and Assembly of a Versatile Lactonase for Bacterial Quorum Quenching

• Published in: Molecules (Basel, Switzerland) Vol. 23; no. 2; p. 341
• Main Authors: Rhoads, Melissa K, Hauk, Pricila, Gupta, Valerie, Bookstaver, Michelle L, Stephens, Kristina, Payne, Gregory F, Bentley, William E
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 06.02.2018; MDPI
• Subjects: Alginic acid; antibiotics; Bacteria; Biodegradation; Biopolymers; C-Terminus; Chemical communication; Chitosan; Communication; Crosstalk; enzyme attachment; enzyme delivery; Enzymes; Glutamine; Homoserine lactones; organophosphate; Pathogenicity; Pathogens; Phosphotriesterase; quorum quenching; Self-assembly; Tyrosine; organophosphate; antibiotics; enzyme delivery; quorum quenching; enzyme attachment

This work sets out to provide a self-assembled biopolymer capsule activated with a multi-functional enzyme for localized delivery. This enzyme, Pox, which is a lactonase and phosphotriesterase, provides a means of interrupting bacterial communication pathways that have been shown to mediate pathogen...