Modification and Assembly of a Versatile Lactonase for Bacterial Quorum Quenching

This work sets out to provide a self-assembled biopolymer capsule activated with a multi-functional enzyme for localized delivery. This enzyme, Pox, which is a lactonase and phosphotriesterase, provides a means of interrupting bacterial communication pathways that have been shown to mediate pathogen...

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Published inMolecules (Basel, Switzerland) Vol. 23; no. 2; p. 341
Main Authors Rhoads, Melissa K, Hauk, Pricila, Gupta, Valerie, Bookstaver, Michelle L, Stephens, Kristina, Payne, Gregory F, Bentley, William E
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 06.02.2018
MDPI
Subjects
Alginic acid
antibiotics
Bacteria
Biodegradation
Biopolymers
C-Terminus
Chemical communication
Chitosan
Communication
Crosstalk
enzyme attachment
enzyme delivery
Enzymes
Glutamine
Homoserine lactones
organophosphate
Pathogenicity
Pathogens
Phosphotriesterase
quorum quenching
Self-assembly
Tyrosine
organophosphate
antibiotics
enzyme delivery
quorum quenching
enzyme attachment
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Summary:This work sets out to provide a self-assembled biopolymer capsule activated with a multi-functional enzyme for localized delivery. This enzyme, Pox, which is a lactonase and phosphotriesterase, provides a means of interrupting bacterial communication pathways that have been shown to mediate pathogenicity. Here we demonstrate the capability to express, purify and attach Pox to the natural biopolymer chitosan, preserving its activity to "neutralize" long-chain autoinducer-1 (AI-1) communication molecules. Attachment is shown via non-specific binding and by engineering tyrosine and glutamine affinity 'tags' at the C-terminus for covalent linkage. Subsequent degradation of AI-1, in this case -(3-oxododecanoyl)-l-homoserine lactone (OdDHL), serves to "quench" bacterial quorum sensing (QS), silencing intraspecies communication. By attaching enzymes to pH-responsive chitosan that, in turn, can be assembled into various forms, we demonstrate device-based flexibility for enzyme delivery. Specifically, we have assembled quorum-quenching capsules consisting of an alginate inner core and an enzyme "decorated" chitosan shell that are shown to preclude bacterial QS crosstalk, minimizing QS mediated behaviors.
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ISSN:1420-3049
1420-3049
DOI:10.3390/molecules23020341