Tetrahydroxystilbene Glucoside Regulates Proliferation, Differentiation, and OPG/RANKL/M-CSF Expression in MC3T3-E1 Cells via the PI3K/Akt Pathway

• Published in: Molecules (Basel, Switzerland) Vol. 23; no. 9; p. 2306
• Main Authors: Fan, Ying-Sai, Li, Qin, Hamdan, Nawras, Bian, Yi-Fei, Zhuang, Shen, Fan, Kai, Liu, Zhong-Jie
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 10.09.2018; MDPI
• Subjects: 1-Phosphatidylinositol 3-kinase; AKT protein; Animals; Biomedical materials; Bone growth; Bone resorption; Bones; Cbfa-1 protein; Cell cycle; Cell Cycle - drug effects; Cell Differentiation - drug effects; Cell growth; Cell Line; Cell proliferation; Cell Proliferation - drug effects; Cell Survival - drug effects; Chinese medicine; Chromones - pharmacology; Collagen (type I); Deoxyribonucleic acid; DNA; DNA - metabolism; DNA biosynthesis; Down-Regulation - drug effects; Down-Regulation - genetics; Glucosides - chemistry; Glucosides - pharmacology; herbal medicine; Herbs; Kinases; Macrophage colony-stimulating factor; Macrophage Colony-Stimulating Factor - genetics; Macrophage Colony-Stimulating Factor - metabolism; Mice; Morpholines - pharmacology; osteoblast; Osteoblasts; Osteoclasts; Osteogenesis; Osteoporosis; Osteoprotegerin; Osteoprotegerin - genetics; Osteoprotegerin - metabolism; Phosphatidylinositol 3-Kinases - metabolism; PI3K/Akt; Protein expression; Proteins; Proto-Oncogene Proteins c-akt - metabolism; RANK Ligand - genetics; RANK Ligand - metabolism; RNA, Messenger - genetics; RNA, Messenger - metabolism; S phase; Side effects; Signal Transduction - drug effects; Stem cells; Stilbenes - chemistry; Stilbenes - pharmacology; tetrahydroxystilbene glucoside; TRANCE protein; Transcription; Transcription factors; Up-Regulation - drug effects; Up-Regulation - genetics; osteoblast; tetrahydroxystilbene glucoside; osteoporosis; herbal medicine; PI3K/Akt
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules23092306

Tetrahydroxystilbene glucoside (TSG) is a unique component of the bone-reinforcing herb Preparata (RPMP). It has the ability to promote bone formation and protect osteoblasts. However, the underlying mechanism remains unclear. To better understand its biological function, we determined TSG's effect on murine pre-osteoblastic MC3T3-E1 cells by the MTT assay, flow cytometry, FQ-PCR, Western blot, and ELISA. The results showed that TSG caused an elevation of the MC3T3-E1 cell number, the number of cells in the S phase, and the mRNA levels of the runt-related transcription factor-2 (Runx2), osterix (Osx), and collagen type I α1 (Col1a1). In addition, the osteoprotegerin (OPG) mRNA level was up-regulated, while the nuclear factor-κB ligand (RANKL) and macrophage colony-stimulating factor (M-CSF) mRNA levels were down-regulated by TSG. Furthermore, TSG activated the phosphoinosmde-3-kinase/protein kinase B (also known as PI3K/Akt) pathway, and blocking this pathway by the inhibitor LY-294002 could impair TSG's functions in relation to the MC3T3-E1 cells. In conclusion, TSG could activate the PI3K/Akt pathway and thus promote MC3T3-E1 cell proliferation and differentiation, and influence OPG/RANKL/M-CSF expression. TSG merits further investigation as a potential therapeutic agent for osteoporosis treatment.