Selenium–Vitamin E Combination and Melatonin Modulates Diabetes-Induced Blood Oxidative Damage and Fetal Outcomes in Pregnant Rats

• Published in: Biological trace element research Vol. 143; no. 2; pp. 1091 - 1102
• Main Authors: Guney, Mehmet, Erdemoglu, Evrim, Mungan, Tamer
• Format: Journal Article
• Language: English
• Published: New York Springer-Verlag 01.11.2011; Humana Press Inc; Springer Nature B.V
• Subjects: administration & dosage; Animal reproduction; Animals; antioxidant activity; Antioxidants; Antioxidants - therapeutic use; Biochemistry; Biomedical and Life Sciences; Biotechnology; Blood; Catalase; Catalase - metabolism; diabetes; Diabetes mellitus; Diabetes Mellitus, Experimental; Diabetes Mellitus, Experimental - drug therapy; Diabetes Mellitus, Experimental - metabolism; Drug Combinations; drug effects; drug therapy; Enzymatic activity; enzyme activity; Enzymes; Female; females; Fetuses; Glucose; Glutathione peroxidase; Glutathione Peroxidase - metabolism; Life Sciences; Lipid peroxidation; Lipid Peroxidation - drug effects; Melatonin; Melatonin - administration & dosage; Melatonin - therapeutic use; metabolism; Nutrition; Obesity; Oncology; Oxidation; Oxidative stress; Oxidative Stress - drug effects; Peroxidation; Pregnancy; Random Allocation; Rats; Rodents; Selenium; Selenium - administration & dosage; Selenium - therapeutic use; Streptozocin; streptozotocin; Superoxide dismutase; Superoxide Dismutase - metabolism; therapeutic use; Vitamin E; Vitamin E - administration & dosage; Vitamin E - therapeutic use; Pregnancy; Oxidative stress; Melatonin; Diabetes; Selenium; Vitamin E
• ISSN: 0163-4984; 1559-0720; 1559-0720
• DOI: 10.1007/s12011-010-8951-3

Oxidative stress is considered to be the main cause of diabetic complications. In the current study, we investigated the effect of selenium–vitamin E combination and melatonin on lipid peroxidation (LPO) and scavenging enzyme activity in the blood of streptozocin (STZ)-induced diabetic pregnant rats. Forty female Wistar rats were randomly divided into five groups. The first and second groups were used as the non-pregnant control and pregnant control groups, respectively. The third group was the pregnant diabetic group. Vitamin E plus selenium and melatonin were administered to the diabetic pregnant rats consisting fourth and fifth groups, respectively. Diabetes was induced on day 0 of the study by STZ. Blood samples were taken from all animals on the 20th day of pregnancy. LPO level was higher in diabetic pregnant rats than in control, although superoxide dismutase, catalase, and glutathione peroxidase activities were lower in diabetic pregnant animals than in control. LPO levels were lower both in the two treatment groups than in the diabetic pregnant rats, whereas selenium–vitamin E combination and melatonin caused a significant increase in the activities of these antioxidant enzymes (p < 0.01). In conclusion, vitamin E plus selenium seems to be a more potent antioxidant compared to melatonin in diabetic pregnant rats. Melatonin did not significantly affect the elevated glucose concentration of diabetic pregnant treated with melatonin group. Vitamin E plus selenium may play a role in preventing diabetes-related diseases of pregnant subjects.