Ultrasound-Assisted Enzymatic Extraction of Adenosine from Vietnamese Cordyceps militaris and Bioactivity Analysis of the Extract

Vietnamese Cordyceps militaris (C. militaris) has long been recognized as one of the most valuable traditional Chinese medicines. In this study, adenosine was extracted from Vietnamese C. militaris by ultrasound-assisted enzymatic extraction method (UAEE) using water as a solvent. Then, the effects...

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Bibliographic Details
Published inJournal of chemistry Vol. 2020; no. 2020; pp. 1 - 10
Main Authors Nguyen Huu, Hieu, Ha, Huynh Ky Phuong, Hoang Minh, Nam, Do, Tran Dat, Phan Le Thao, My, Nguyen Cao, Phuc, Vuong Hoai, Thanh, Mai Thanh, Phong
Format Journal Article
LanguageEnglish
Published Cairo, Egypt Hindawi Publishing Corporation 2020
Hindawi
Hindawi Limited
Wiley
Subjects
Acids
Adenosine
Analysis
Antioxidants
Antioxidants (Nutrients)
Biological activity
Cancer
Enzymes
High performance liquid chromatography
Influence
Methods
Performance evaluation
Response surface methodology
Traditional Chinese medicine
Ultrasonic imaging
Ultrasonic testing
Ultrasound
Vietnam
China
United Arab Emirates
United States > US
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Summary:Vietnamese Cordyceps militaris (C. militaris) has long been recognized as one of the most valuable traditional Chinese medicines. In this study, adenosine was extracted from Vietnamese C. militaris by ultrasound-assisted enzymatic extraction method (UAEE) using water as a solvent. Then, the effects of five single factors on adenosine content including pH, enzyme-to-material ratio, ultrasonic power, ultrasonic time, and ultrasonic temperature were determined. After that, three factors consisting of ultrasonic power, ultrasonic time, and ultrasonic temperature were chosen based on their effects on adenosine content. The simultaneous influence of these factors on the adenosine content was investigated by response surface method using central composite design. The adenosine content was evaluated by high-performance liquid chromatography method. Under the optimal conditions, the extract was evaluated for antioxidant and anticancer bioactivities. In addition, different extraction methods including aqueous extraction (AE), ultrasound-assisted extraction (UAE), and enzyme-assisted extraction (EAE) methods were carried out to compare with UAEE. As a result, it can be concluded that UAEE is a promising method for adenosine extraction and further studies regarding isolation and purification need to be conducted.
ISSN:2090-9063
2090-9071
DOI:10.1155/2020/1487654