REBASE--a database for DNA restriction and modification: enzymes, genes and genomes

• Published in: Nucleic acids research Vol. 43; no. Database issue; pp. D298 - D299
• Main Authors: Roberts, Richard J, Vincze, Tamas, Posfai, Janos, Macelis, Dana
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 28.01.2015
• Subjects: Database Issue; Databases, Genetic; DNA Modification Methylases - chemistry; DNA Modification Methylases - genetics; DNA Modification Methylases - metabolism; DNA Restriction Enzymes - chemistry; DNA Restriction Enzymes - genetics; DNA Restriction Enzymes - metabolism; Genes; Genomics; Internet; Sequence Analysis, DNA; Software
• ISSN: 0305-1048; 1362-4962
• DOI: 10.1093/nar/gku1046

REBASE is a comprehensive and fully curated database of information about the components of restriction-modification (RM) systems. It contains fully referenced information about recognition and cleavage sites for both restriction enzymes and methyltransferases as well as commercial availability, methylation sensitivity, crystal and sequence data. All genomes that are completely sequenced are analyzed for RM system components, and with the advent of PacBio sequencing, the recognition sequences of DNA methyltransferases (MTases) are appearing rapidly. Thus, Type I and Type III systems can now be characterized in terms of recognition specificity merely by DNA sequencing. The contents of REBASE may be browsed from the web http://rebase.neb.com and selected compilations can be downloaded by FTP (ftp.neb.com). Monthly updates are also available via email.