Analysis of indole-3-acetic acid metabolites from Dalbergia dolichopetala by high performance liquid chromatography-mass spectrometry

• Published in: Plant physiology (Bethesda) Vol. 100; no. 1; pp. 63 - 68
• Main Authors: Ostin, A, Monteiro, A.M, Crozier, A, Jensen, E, Sandberg, G
• Format: Journal Article
• Language: English
• Published: Rockville, MD American Society of Plant Physiologists 01.09.1992
• Subjects: Acetates; ACIDE INDOLACETIQUE; ACIDO INDOLACETICO; Amino acid metabolism; Biological and medical sciences; CHROMATOGRAPHIE LIQUIDE HAUTE PRESS; COTILEDONES; COTYLEDON; Cotyledons; CROMATOGRAFIA LIQUIDA ALTA PRESION; DALBERGIA; Development and Growth Regulation; ESPECTROMETRIA DE MASAS; Fundamental and applied biological sciences. Psychology; GRAINE; Growth regulators; INDOL; INDOLES; Liquids; Mass spectra; Mass spectrometers; Metabolism; METABOLISME; METABOLISMO; METABOLITE; Metabolites; METABOLITOS; Plant physiology and development; Plants; Radioactive decay; Seedlings; SEMILLA; SPECTROMETRIE DE MASSE; Auxin; Seeds; Radiolabelling; Metabolite; Germination; HPLC chromatography; Metabolism; Chromatography; Spectrometry; Leguminosae; Dicotyledones; Angiospermae; Plant growth substance; Isolation; Spermatophyta; Mass spectrometry
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.100.1.63

A mixture of [2-14C1] and [13C6]indole-3-acetic acid was applied to the cotyledons of 6-day-germinated seeds of "jacaranda do cerrado" (Dalbergia dolichopetala) and after 8 hours the seeds were extracted. Analysis of the fractionated extract by reversed-phase high performance liquid chromatography-radiocounting revealed the presence of five radiolabeled metabolite peaks (I-V). After further purification, the individual peaks of radioactivity were analyzed by combined high performance liquid chromatography-steel filter-fast atom bombardment-mass spectrometry. The metabolite fraction V was found to contain [14C1,13C6]indole-3-acetylaspartic acid and unlabeled indole-3-acetylglutamic acid. Analysis of the metabolite fraction II revealed the presence of dioxindole-3-acetylaspartic acid and putative dioxindole-3-acetylglutamic acid as well as putative benzene ring-hydroxylated derivatives of oxindole-3-acetylaspartic acid and oxindole-3-acetylglutamic acid. There was no evidence of significant incorporation of label from [2'-14C1] or [13C6]indole-3-acetic acid into any of these conjugated indoles