Trehalose-6-phosphate promotes fermentation and glucose repression in Saccharomyces cerevisiae
The yeast trehalose-6-phosphate synthase (Tps1) catalyzes the formation of trehalose-6-phosphate (T6P) in trehalose synthesis. Besides, Tps1 plays a key role in carbon and energy homeostasis in this microbial cell, as shown by the well documented loss of ATP and hyper accumulation of sugar phosphate...
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Published in | Microbial cell Vol. 5; no. 10; pp. 444 - 459 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
Austria
Shared Science Publishers OG
01.10.2018
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Subjects | |
Online Access | Get full text |
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Summary: | The yeast trehalose-6-phosphate synthase (Tps1) catalyzes the formation of trehalose-6-phosphate (T6P) in trehalose synthesis. Besides, Tps1 plays a key role in carbon and energy homeostasis in this microbial cell, as shown by the well documented loss of ATP and hyper accumulation of sugar phosphates in response to glucose addition in a mutant defective in this protein. The inability of a
mutant to cope with fermentable sugars is still a matter of debate. We reexamined this question through a quantitative analysis of the capability of
homologues from different origins to complement phenotypic defects of this mutant. Our results allowed to classify this complementation in three groups. A first group enclosed
of
with that of
as their expression in
cells fully recovered wild type metabolic patterns and fermentation capacity in response to glucose. At the opposite was the group with
homologues from the bacteria
and
, the plant
and the insect
whose metabolic profiles were comparable to those of a
mutant, notably with almost no accumulation of T6P, strong impairment of ATP recovery and potent reduction of fermentation capacity, albeit these homologous genes were able to rescue growth of Sc
on glucose. In between was a group consisting of
homologues from other yeast species and filamentous fungi characterized by 5 to 10 times lower accumulation of T6P, a weaker recovery of ATP and a 3-times lower fermentation capacity than wild type. Finally, we found that glucose repression of gluconeogenic genes was strongly dependent on T6P. Altogether, our results suggest that the TPS protein is indispensable for growth on fermentable sugars, and points to a critical role of T6P as a sensing molecule that promotes sugar fermentation and glucose repression. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Conflict of interest: The authors declare that they have no competing interests. Please cite this article as: Rebeca L. Vicente, Lucie Spina, Jose P.L. Gómez, Sebastien Dejean, Jean-Luc Parrou and Jean Marie François (2018). Trehalose-6-phosphate promotes fermentation and glucose repression in Saccharomyces cerevisiae. Microbial Cell 5(10): 444-459. doi: 10.15698/mic2018.10.651 |
ISSN: | 2311-2638 2311-2638 |
DOI: | 10.15698/mic2018.10.651 |