Magnesium-Free Immobilization of DNA Origami Nanostructures at Mica Surfaces for Atomic Force Microscopy

• Published in: Molecules (Basel, Switzerland) Vol. 26; no. 16; p. 4798
• Main Authors: Xin, Yang, Zargariantabrizi, Amir Ardalan, Grundmeier, Guido, Keller, Adrian
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 07.08.2021; MDPI
• Subjects: Adsorption; Aluminum Silicates - chemistry; Atomic force microscopy; Cations; Deoxyribonucleic acid; DNA; DNA - chemistry; DNA nanotechnology; DNA origami; Immobilization; Lysine; Magnesium; Magnesium - chemistry; Mica; Microscopy; Microscopy, Atomic Force; Nanostructures - chemistry; Nickel - chemistry; Nucleic Acid Conformation; Poly-L-lysine; Polyelectrolytes - chemistry; Polylysine - chemistry; Spermidine; Spermidine - chemistry; Substrates; Surface chemistry; Surface Properties; Water - chemistry; DNA nanotechnology; mica; adsorption; DNA origami; atomic force microscopy
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules26164798

DNA origami nanostructures (DONs) are promising substrates for the single-molecule investigation of biomolecular reactions and dynamics by in situ atomic force microscopy (AFM). For this, they are typically immobilized on mica substrates by adding millimolar concentrations of Mg ions to the sample solution, which enable the adsorption of the negatively charged DONs at the like-charged mica surface. These non-physiological Mg concentrations, however, present a serious limitation in such experiments as they may interfere with the reactions and processes under investigation. Therefore, we here evaluate three approaches to efficiently immobilize DONs at mica surfaces under essentially Mg -free conditions. These approaches rely on the pre-adsorption of different multivalent cations, i.e., Ni , poly-l-lysine (PLL), and spermidine (Spdn). DON adsorption is studied in phosphate-buffered saline (PBS) and pure water. In general, Ni shows the worst performance with heavily deformed DONs. For 2D DON triangles, adsorption at PLL- and in particular Spdn-modified mica may outperform even Mg -mediated adsorption in terms of surface coverage, depending on the employed solution. For 3D six-helix bundles, less pronounced differences between the individual strategies are observed. Our results provide some general guidance for the immobilization of DONs at mica surfaces under Mg -free conditions and may aid future in situ AFM studies.