ELICITING AN IMMUNE RESPONSE BY PLASMID DNA ENCODING A HUMAN SPERM PROTEIN (HSD-1)

• Published in: Archives of andrology Vol. 42; no. 3; pp. 127 - 136
• Main Authors: WU, Y.W., CHEN, D.H., MIAO, S. Y., WANG, L.F., ZONG, S. D., KOIDE, S.S.
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA Informa UK Ltd 1999; Taylor & Francis
• Subjects: Animals; Antigens, Surface; Biological and medical sciences; Connective Tissue - metabolism; Cytotoxic t Lymphocytes;Dna Vaccine;Fertility;Sperm Component; Female; Fertility; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Gene Transfer Techniques; Genetics of the immune response; Humans; Immunobiology; In Situ Hybridization; Male; Membrane Proteins - biosynthesis; Membrane Proteins - genetics; Membrane Proteins - immunology; Mice; Molecular Sequence Data; Muscle, Skeletal - metabolism; Phagocytes - cytology; Plasmids - immunology; Spermatozoa - metabolism; Spleen - immunology; T-Lymphocytes, Cytotoxic - cytology; T-Lymphocytes, Cytotoxic - immunology; Transcription, Genetic; Human; Spermatozoa; Immune response; Rodentia; Cytotoxicity; Vaccine; Germinal cell; Gene expression; Intramuscular administration; Plasmid; Vertebrata; Mammalia; Mouse; DNA; T-Lymphocyte; Fecundity
• ISSN: 0955-3002; 0148-5016; 1362-3095; 1521-0375
• DOI: 10.1080/014850199262788

The cDNA encoding a human sperm membrane designated as HSD-1 was isolated from a human testis lambdagt11 cDNA expression library and assigned the accession number U12978 by GenBank. HSD1 was conjugated to an eukaryotic expression plasmid (pRSV) to construct the recombinant plasmid pRSV-HSD-1. Female mice were inoculated intramuscularly with the plasmid DNA and the expression of HSD-1 was determined. HSD-1 mRNAs were detected in myocytes and endomysial connective tissue cells of the quadriceps muscle by in situ hybridization. Spleen of inoculated animals contained an increased number of cytotoxic T lymphocytes, phagocytes, and plasma cells. Fertility of the treated animals was not affected. Thus, intramuscular inoculation of female mice with the plasmid DNA (pRSV-HSD-1) results in the expression of HSD-1 and may elicit a tissue-mediated immune response.