Cancer Cell Membrane-Coated Nanosuspensions for Enhanced Chemotherapeutic Treatment of Glioma

• Published in: Molecules (Basel, Switzerland) Vol. 26; no. 16; p. 5103
• Main Authors: Fan, Yueyue, Hao, Wenyan, Cui, Yuexin, Chen, Mengyu, Chu, Xiaoyang, Yang, Yang, Wang, Yuli, Gao, Chunsheng
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 23.08.2021; MDPI
• Subjects: Animals; Antineoplastic Agents, Phytogenic - adverse effects; Antineoplastic Agents, Phytogenic - pharmacology; Antineoplastic Agents, Phytogenic - therapeutic use; biomimetic drug delivery system; Biomimetics; Blood-brain barrier; Blood-Brain Barrier - drug effects; Blood-Brain Barrier - pathology; Brain cancer; Brain Neoplasms - drug therapy; Brain Neoplasms - pathology; Brain tumors; Camptothecin - adverse effects; Camptothecin - analogs & derivatives; Camptothecin - pharmacology; Camptothecin - therapeutic use; Cancer; cancer cell membrane; Cancer therapies; Cell Line, Tumor; Cell Membrane - pathology; Cell membranes; Chemical properties; Drug delivery; Drug delivery systems; Drugs; Endocytosis - drug effects; Experiments; Female; Glioma; Glioma - drug therapy; Glioma - pathology; Glioma cells; Homogenization; Homology; homotypic targeting; Humans; Immune system; Immunosuppressive agents; Male; Medical research; Membranes; Mice; Mice, Inbred ICR; Morphology; Nanomaterials; Nanoparticles; Nanoparticles - chemistry; Nanoparticles - ultrastructure; nanosuspensions; Neuroimaging; Particle size; Permeability; Rats; Suspensions; Tissue Distribution - drug effects; Treatment Outcome; Tumors; Beijing China; China; nanosuspensions; glioma; homotypic targeting; biomimetic drug delivery system; cancer cell membrane
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules26165103

Effective intracerebral delivery is key for glioma treatment. However, the drug delivery system within the brain is largely limited by its own adverse physical and chemical properties, low targeting efficiency, the blood-brain barrier and the blood-brain tumor barrier. Herein, we developed a simple, safe and efficient biomimetic nanosuspension. The C6 cell membrane (CCM) was utilized to camouflaged the 10-hydroxycamptothecin nanosuspension (HCPT-NS) in order to obtain HCPT-NS/CCM. Through the use of immune escape and homotypic binding of the cancer cell membrane, HCPT-NS/CCM was able to penetrate the blood-brain barrier and target tumors. The HCPT-NS is only comprised of drugs, as well as a small amount of stabilizers that are characterized by a simple preparation method and high drug loading. Similarly, the HCPT-NS/CCM is able to achieve targeted treatment of glioma without any ligand modification, which leads it to be stable and efficient. Cellular uptake and in vivo imaging experiments demonstrated that HCPT-NS/CCM is able to effectively cross the blood-brain barrier and was concentrated at the glioma site due to the natural homing pathway. Our results reveal that the glioma cancer cell membrane is able to promote drug transport into the brain and enter the tumor via a homologous targeting mechanism.