Mammillaria Species-Polyphenols Studies and Anti-Cancer, Anti-Oxidant, and Anti-Bacterial Activities

• Published in: Molecules (Basel, Switzerland) Vol. 25; no. 1; p. 131
• Main Authors: O Elansary, Hosam, Szopa, Agnieszka, Klimek-Szczykutowicz, Marta, Jafernik, Karolina, Ekiert, Halina, Mahmoud, Eman A, Abdelmoneim Barakat, Ahmed, O El-Ansary, Diaa
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 29.12.2019; MDPI
• Subjects: Acids; anti-bacterial; anti-cancer; anti-oxidant; Antibacterial activity; Antioxidants; Antiproliferatives; Apoptosis; Bacteria; Bacterial infections; Bleaching; Brain cancer; Caffeic acid; Cancer; Carotene; Cell cycle; Chlorogenic acid; cytotoxicity; Dihydroxybenzoic acid; Enzymes; Flavonoids; Flowers & plants; Gardens & gardening; Gentisic acid; High performance liquid chromatography; Kinases; Liquid chromatography; Mammillaria; Metabolites; Natural products; Natural resources; Oxidants; Oxidizing agents; p-Hydroxybenzoic acid; Phenolic acids; Phenols; Polyphenols; Protocatechuic acid; Sinapic acid; stem extract; Tumors; β-Carotene; anti-bacterial; Mammillaria; phenolic acids; anti-oxidant; stem extract; anti-cancer; cytotoxicity
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules25010131

Discovering new natural resources of polyphenols is the aim of many recent studies in the field of natural product research. This study tentatively investigated the polyphenols profile of the stems of seven species , and ) using high performance liquid chromatography with DAD detector (HPLC-DAD) method. Furthermore, the anti-cancer, anti-oxidant, and anti-bacterial potentials of these extracts as well as major identified phenols were explored. The HPLC-DAD study confirmed the availability of six phenolic acids, including gentisic acid, chlorogenic acid, caffeic acid, protocatechuic acid, sinapic acid, and p-hydroxybenzoic acid. The dominant compounds were: gentisic acid in and ; chlorogenic acid in , and and caffeic acid in and Stems of sp. showed antiproliferative effects against HeLa, MCF-7, and Jurkat cells. In HeLa and MCF-7 cells, the best antiproliferative activities were found in the treatments with , and . The apoptotic assay of , and showed accumulation of necrotic cells in the early and late apoptotic phase. , and showed the highest anti-oxidant activities using 2,2-diphenyl-1-picrylhydrazyl (DPPH), β-carotene bleaching, and ferric reducing anti-oxidant power (FRAP) assays. was the best source of antioxidants. sp. showed moderate anti-bacterial effects against bacteria and the highest effects were found using the extracts of and against most bacteria. The anti-bacterial activities were attributed to other phenolic compounds (e.g., chlorogenic acid) than gentisic acid, which was not active against most bacteria. sp. could be considered to be an important natural source of phenolic acids with anti-cancer, anti-bacterial, and anti-oxidant activities.