Clostridium acetobutylicum grows vegetatively in a biofilm rich in heteropolysaccharides and cytoplasmic proteins

• Published in: Biotechnology for biofuels Vol. 11; no. 1; p. 315
• Main Authors: Liu, Dong, Yang, Zhengjiao, Chen, Yong, Zhuang, Wei, Niu, Huanqing, Wu, Jinglan, Ying, Hanjie
• Format: Journal Article
• Language: English
• Published: England BioMed Central 20.11.2018; BMC
• Subjects: Adhesins; Bacteria; Biodiesel fuels; Biofilm; Biofilms; Biofuels; Biosynthesis; Clostridium acetobutylicum; Composition; Cotton; Gene expression; Glucose; Kinases; Mannose; Metabolism; Moonlighting protein; Organic chemistry; Phenotypes; Physiology; Polysaccharide; Polysaccharides; Proteins; Saccharides; Solvents; Sporulation; Stainless steel; Biofilm; Clostridium acetobutylicum; Moonlighting protein; Sporulation; Polysaccharide
• ISSN: 1754-6834; 1754-6834
• DOI: 10.1186/s13068-018-1316-4

Biofilms are cell communities wherein cells are embedded in a self-produced extracellular polymeric substances (EPS). The biofilm of confers the cells superior phenotypes and has been extensively exploited to produce a variety of liquid biofuels and bulk chemicals. However, little has been known about the physiology of in biofilm as well as the composition and biosynthesis of the EPS. Thus, this study is focused on revealing the cell physiology and EPS composition of biofilm. Here, we revealed a novel lifestyle of in biofilm: elimination of sporulation and vegetative growth. Extracellular polymeric substances and wire-like structures were also observed in the biofilm. Furthermore, for the first time, the biofilm polysaccharides and proteins were isolated and characterized. The biofilm contained three heteropolysaccharides. The major fraction consisted of predominantly glucose, mannose and aminoglucose. Also, a great variety of proteins including many non-classically secreted proteins moonlighting as adhesins were found considerably present in the biofilm, with GroEL, a S-layer protein and rubrerythrin being the most abundant ones. This study evidenced that vegetative cells rather than commonly assumed spore-forming cells were essentially the solvent-forming cells. The abundant non-classically secreted moonlighting proteins might be important for the biofilm formation. This study provides the first physiological and molecular insights into biofilm which should be valuable for understanding and development of the biofilm-based processes.