Bauhinia forficata Link Infusions: Chemical and Bioactivity of Volatile and Non-Volatile Fractions

• Published in: Molecules (Basel, Switzerland) Vol. 27; no. 17; p. 5415
• Main Authors: Jung, Eliane Przytyk, de Freitas, Beatriz Pereira, Kunigami, Claudete Norie, Moreira, Davyson de Lima, de Figueiredo, Natália Guimarães, Ribeiro, Leilson de Oliveira, Moreira, Ricardo Felipe Alves
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 24.08.2022; MDPI
• Subjects: alpha-Amylases; Amylases; antioxidant capacity; Antioxidants; Antioxidants - chemistry; Bauhinia; Bauhinia - chemistry; Bioactive compounds; Biological activity; Brazil; Commercialization; Diabetes; Flavonoids; Flavonoids - pharmacology; Folk medicine; Herbal medicine; Heterogeneity; pata-de-vaca; Phenolic compounds; Phenols; phytochemical profile; Phytochemicals; Plant Extracts - chemistry; Rheumatism; SPME technique; Uric acid; Volatile compounds; α-Amylase; α-amylase inhibition; Brazil; phytochemical profile; SPME technique; bioactive compounds; pata-de-vaca; α-amylase inhibition; antioxidant capacity
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules27175415

This study aimed to evaluate Bauhinia forficata infusions prepared using samples available in Rio de Janeiro, Brazil. As such, infusions at 5% (w/v) of different brands and batches commercialized in the city (CS1, CS2, CS3, and CS4) and samples of plant material botanically identified (BS) were evaluated to determine their total phenolic and flavonoid contents (TPC and TFC), antioxidant capacity (ABTS•+, DPPH•, and FRAP assays), phytochemical profile, volatile compounds, and inhibitory effects against the α-amylase enzyme. The results showed that infusions prepared using BS samples had lower TPC, TFC and antioxidant potential than the commercial samples (p < 0.05). The batch averages presented high standard deviations mainly for the commercial samples, corroborating sample heterogeneity. Sample volatile fractions were mainly composed of terpenes (40 compounds identified). In the non-volatile fraction, 20 compounds were identified, with emphasis on the CS3 sample, which comprised most of the compounds, mainly flavonoid derivatives. PCA analysis demonstrated more chemical diversity in non-volatile than volatile compounds. The samples also inhibited the α-amylase enzyme (IC50 value: 0.235−0.801 mg RE/mL). Despite the differences observed in this work, B. forficata is recognized as a source of bioactive compounds that can increase the intake of antioxidant compounds by the population.