Green Synthesis, Characterization, and Antibacterial Properties of Silver Nanoparticles Obtained by Using Diverse Varieties of Cannabis sativa Leaf Extracts

• Published in: Molecules (Basel, Switzerland) Vol. 26; no. 13; p. 4041
• Main Authors: Csakvari, Adriana Cecilia, Moisa, Cristian, Radu, Dana G, Olariu, Leonard M, Lupitu, Andreea I, Panda, Anca Ofelia, Pop, Georgeta, Chambre, Dorina, Socoliuc, Vlad, Copolovici, Lucian, Copolovici, Dana Maria
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.07.2021; MDPI
• Subjects: Alzheimer's disease; Anti-Bacterial Agents - chemistry; Antibacterial activity; antioxidant activity; Antioxidants; Antioxidants - chemistry; Aqueous solutions; Assaying; Biosynthesis; Cannabinoids; Cannabis; Cannabis - chemistry; Cannabis sativa; Chemical composition; Flavonoids; Food production; Fourier transforms; Gallic acid; Green buildings; Green Chemistry Technology - methods; Green development; green synthesis; Hemp; High performance liquid chromatography; Infrared spectroscopy; Klebsiella; Leaves; Light scattering; Liquid chromatography; Marijuana; Mass spectrometry; Mass spectroscopy; Medical treatment; Metabolites; Metal Nanoparticles - chemistry; Nanoparticles; Particle size; Photon correlation spectroscopy; Phytochemicals; Phytoremediation; Plant extracts; Plant Extracts - chemistry; Plant Leaves - chemistry; Polyphenols - analysis; Pseudomonas fluorescens; Scanning electron microscopy; Seeds; SEM-EDX; Silver; Silver - chemistry; silver nanoparticles; Sulfonic acid; Sustainable materials; Synergistic effect; Textile industry; Ultrasonic imaging; antibacterial activity; SEM-EDX; Cannabis sativa; UHPLC-DAD-MS; silver nanoparticles; green synthesis; antioxidant activity
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules26134041

L. (hemp) is a plant used in the textile industry and green building material industry, as well as for the phytoremediation of soil, medical treatments, and supplementary food products. The synergistic effect of terpenes, flavonoids, and cannabinoids in hemp extracts may mediate the biogenic synthesis of metal nanoparticles. In this study, the chemical composition of aqueous leaf extracts of three varieties of Romanian hemp (two monoecious, and one dioecious) have been determined by Fourier-Transformed Infrared spectroscopy (FT-IR), high-performance liquid chromatography, and mass spectrometry (UHPLC-DAD-MS). Then, their capability to mediate the green synthesis of silver nanoparticles (AgNPs) and their pottential antibacterial applications were evaluated. The average antioxidant capacity of the extracts had 18.4 ± 3.9% inhibition determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH ) and 78.2 ± 4.1% determined by 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS™) assays. The total polyphenolic content of the extracts was 1642 ± 32 mg gallic acid equivalent (GAE) L . After this, these extracts were reacted with an aqueous solution of AgNO resulting in AgNPs, which were characterized by UV-VIS spectroscopy, FT-IR, scanning electron microscopy (SEM-EDX), and dynamic light scattering (DLS). The results demonstrated obtaining spherical, stable AgNPs with a diameter of less than 69 nm and an absorbance peak at 435 nm. The mixture of extracts and AgNPs showed a superior antioxidant capacity of 2.3 ± 0.4% inhibition determined by the DPPH assay, 88.5 ± 0.9% inhibition as determined by the ABTS assay, and a good antibacterial activity against several human pathogens: and